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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Flavobacterium johnsoniae 30S ribosomal protein S6 (rpsF) CSB-YP399127FDT
CSB-EP399127FDT
CSB-BP399127FDT
CSB-MP399127FDT
CSB-EP399127FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Probable DNA repair protein Fjoh_3304 (Fjoh_3304) CSB-YP399128FDT
CSB-EP399128FDT
CSB-BP399128FDT
CSB-MP399128FDT
CSB-EP399128FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae UPF0173 metal-dependent hydrolase Fjoh_2786 (Fjoh_2786) CSB-YP399129FDT
CSB-EP399129FDT
CSB-BP399129FDT
CSB-MP399129FDT
CSB-EP399129FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Phosphopantetheine adenylyltransferase (coaD) CSB-YP399130FDT
CSB-EP399130FDT
CSB-BP399130FDT
CSB-MP399130FDT
CSB-EP399130FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP399131FDT
CSB-EP399131FDT
CSB-BP399131FDT
CSB-MP399131FDT
CSB-EP399131FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Adenylosuccinate synthetase (purA) CSB-YP399132FDT
CSB-EP399132FDT
CSB-BP399132FDT
CSB-MP399132FDT
CSB-EP399132FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 50S ribosomal protein L7/L12 (rplL) CSB-YP399133FDT
CSB-EP399133FDT
CSB-BP399133FDT
CSB-MP399133FDT
CSB-EP399133FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 30S ribosomal protein S21 (rpsU) CSB-YP399134FDT
CSB-EP399134FDT
CSB-BP399134FDT
CSB-MP399134FDT
CSB-EP399134FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP399135FDT
CSB-EP399135FDT
CSB-BP399135FDT
CSB-MP399135FDT
CSB-EP399135FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Probable GTP-binding protein EngB (engB) CSB-YP399136FDT
CSB-EP399136FDT
CSB-BP399136FDT
CSB-MP399136FDT
CSB-EP399136FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP399137FDT
CSB-EP399137FDT
CSB-BP399137FDT
CSB-MP399137FDT
CSB-EP399137FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP399138FDT
CSB-EP399138FDT
CSB-BP399138FDT
CSB-MP399138FDT
CSB-EP399138FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 50S ribosomal protein L27 (rpmA) CSB-YP399139FDT
CSB-EP399139FDT
CSB-BP399139FDT
CSB-MP399139FDT
CSB-EP399139FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii 50S ribosomal protein L19 (rplS) CSB-YP399140VDY
CSB-EP399140VDY
CSB-BP399140VDY
CSB-MP399140VDY
CSB-EP399140VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii Translation initiation factor IF-1 (infA) CSB-YP399141VDY
CSB-EP399141VDY
CSB-BP399141VDY
CSB-MP399141VDY
CSB-EP399141VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii D-alanine--D-alanine ligase (ddl) CSB-YP399142VDY
CSB-EP399142VDY
CSB-BP399142VDY
CSB-MP399142VDY
CSB-EP399142VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP399143VDY
CSB-EP399143VDY
CSB-BP399143VDY
CSB-MP399143VDY
CSB-EP399143VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii Alanine racemase (alr) CSB-YP399144VDY
CSB-EP399144VDY
CSB-BP399144VDY
CSB-MP399144VDY
CSB-EP399144VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii Threonine--tRNA ligase (thrS), partial CSB-YP399145VDY
CSB-EP399145VDY
CSB-BP399145VDY
CSB-MP399145VDY
CSB-EP399145VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP399146VDY
CSB-EP399146VDY
CSB-BP399146VDY
CSB-MP399146VDY
CSB-EP399146VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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