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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Metallosphaera sedula Elongation factor 1-beta (ef1b) CSB-YP398046MNM
CSB-EP398046MNM
CSB-BP398046MNM
CSB-MP398046MNM
CSB-EP398046MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP398047MNM
CSB-EP398047MNM
CSB-BP398047MNM
CSB-MP398047MNM
CSB-EP398047MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula 50S ribosomal protein L39e (rpl39e) CSB-YP398048MNM
CSB-EP398048MNM
CSB-BP398048MNM
CSB-MP398048MNM
CSB-EP398048MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Alanine--tRNA ligase (alaS), partial CSB-YP398049MNM
CSB-EP398049MNM
CSB-BP398049MNM
CSB-MP398049MNM
CSB-EP398049MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Acylphosphatase (acyP) CSB-YP398050MNM
CSB-EP398050MNM
CSB-BP398050MNM
CSB-MP398050MNM
CSB-EP398050MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula DNA-directed RNA polymerase subunit L (rpoL) CSB-YP398051MNM
CSB-EP398051MNM
CSB-BP398051MNM
CSB-MP398051MNM
CSB-EP398051MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP398052MNM
CSB-EP398052MNM
CSB-BP398052MNM
CSB-MP398052MNM
CSB-EP398052MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula ATP phosphoribosyltransferase (hisG) CSB-YP398053MNM
CSB-EP398053MNM
CSB-BP398053MNM
CSB-MP398053MNM
CSB-EP398053MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP398054MNM
CSB-EP398054MNM
CSB-BP398054MNM
CSB-MP398054MNM
CSB-EP398054MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Protein Msed_2121 (Msed_2121) CSB-YP398055MNM
CSB-EP398055MNM
CSB-BP398055MNM
CSB-MP398055MNM
CSB-EP398055MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula 30S ribosomal protein S2P (rps2p) CSB-YP398056MNM
CSB-EP398056MNM
CSB-BP398056MNM
CSB-MP398056MNM
CSB-EP398056MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula 30S ribosomal protein S24e (rps24e) CSB-YP398057MNM
CSB-EP398057MNM
CSB-BP398057MNM
CSB-MP398057MNM
CSB-EP398057MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Transcription factor E (tfe) CSB-YP398058MNM
CSB-EP398058MNM
CSB-BP398058MNM
CSB-MP398058MNM
CSB-EP398058MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP398059BPF
CSB-EP398059BPF
CSB-BP398059BPF
CSB-MP398059BPF
CSB-EP398059BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP398060BPF
CSB-EP398060BPF
CSB-BP398060BPF
CSB-MP398060BPF
CSB-EP398060BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Integration host factor subunit beta (ihfB) CSB-YP398061BPF
CSB-EP398061BPF
CSB-BP398061BPF
CSB-MP398061BPF
CSB-EP398061BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP398062BPF
CSB-EP398062BPF
CSB-BP398062BPF
CSB-MP398062BPF
CSB-EP398062BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. UPF0102 protein BRADO0179 (BRADO0179) CSB-YP398063BPF
CSB-EP398063BPF
CSB-BP398063BPF
CSB-MP398063BPF
CSB-EP398063BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP398064BPF
CSB-EP398064BPF
CSB-BP398064BPF
CSB-MP398064BPF
CSB-EP398064BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Probable intracellular septation protein A (BRADO0387), partial CSB-YP398065BPF1
CSB-EP398065BPF1
CSB-BP398065BPF1
CSB-MP398065BPF1
CSB-EP398065BPF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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