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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bradyrhizobium sp. ATP synthase gamma chain (atpG) CSB-YP398066BPF
CSB-EP398066BPF
CSB-BP398066BPF
CSB-MP398066BPF
CSB-EP398066BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L27 (rpmA) CSB-YP398067BPF
CSB-EP398067BPF
CSB-BP398067BPF
CSB-MP398067BPF
CSB-EP398067BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP398068BPF
CSB-EP398068BPF
CSB-BP398068BPF
CSB-MP398068BPF
CSB-EP398068BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L28 (rpmB) CSB-YP398069BPF
CSB-EP398069BPF
CSB-BP398069BPF
CSB-MP398069BPF
CSB-EP398069BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Urease accessory protein UreD 1 (ureD1) CSB-YP398070BPF
CSB-EP398070BPF
CSB-BP398070BPF
CSB-MP398070BPF
CSB-EP398070BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Probable potassium transport system protein kup 1 (kup1), partial CSB-YP398071BPF
CSB-EP398071BPF
CSB-BP398071BPF
CSB-MP398071BPF
CSB-EP398071BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Light-independent protochlorophyllide reductase subunit N (bchN) CSB-YP398072BPF
CSB-EP398072BPF
CSB-BP398072BPF
CSB-MP398072BPF
CSB-EP398072BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP398073BPF
CSB-EP398073BPF
CSB-BP398073BPF
CSB-MP398073BPF
CSB-EP398073BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP398074BPF
CSB-EP398074BPF
CSB-BP398074BPF
CSB-MP398074BPF
CSB-EP398074BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L11 (rplK) CSB-YP398075BPF
CSB-EP398075BPF
CSB-BP398075BPF
CSB-MP398075BPF
CSB-EP398075BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L4 (rplD) CSB-YP398076BPF
CSB-EP398076BPF
CSB-BP398076BPF
CSB-MP398076BPF
CSB-EP398076BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L22 (rplV) CSB-YP398077BPF
CSB-EP398077BPF
CSB-BP398077BPF
CSB-MP398077BPF
CSB-EP398077BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L30 (rpmD) CSB-YP398078BPF
CSB-EP398078BPF
CSB-BP398078BPF
CSB-MP398078BPF
CSB-EP398078BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Octanoyltransferase (lipB) CSB-YP398079BPF
CSB-EP398079BPF
CSB-BP398079BPF
CSB-MP398079BPF
CSB-EP398079BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 30S ribosomal protein S18 (rpsR) CSB-YP398080BPF
CSB-EP398080BPF
CSB-BP398080BPF
CSB-MP398080BPF
CSB-EP398080BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. C4-dicarboxylate transport protein 2 (dctA2), partial CSB-YP398081BPF
CSB-EP398081BPF
CSB-BP398081BPF
CSB-MP398081BPF
CSB-EP398081BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 1-aminocyclopropane-1-carboxylate deaminase (acdS) CSB-YP398082BPF
CSB-EP398082BPF
CSB-BP398082BPF
CSB-MP398082BPF
CSB-EP398082BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 5'-nucleotidase surE (surE) CSB-YP398083BPF
CSB-EP398083BPF
CSB-BP398083BPF
CSB-MP398083BPF
CSB-EP398083BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP398084BPF
CSB-EP398084BPF
CSB-BP398084BPF
CSB-MP398084BPF
CSB-EP398084BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Proline--tRNA ligase (proS) CSB-YP398085BPF
CSB-EP398085BPF
CSB-BP398085BPF
CSB-MP398085BPF
CSB-EP398085BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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