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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bradyrhizobium sp. NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP398086BPF
CSB-EP398086BPF
CSB-BP398086BPF
CSB-MP398086BPF
CSB-EP398086BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP398087BPF
CSB-EP398087BPF
CSB-BP398087BPF
CSB-MP398087BPF
CSB-EP398087BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. DNA repair protein recO (recO) CSB-YP398088BPF
CSB-EP398088BPF
CSB-BP398088BPF
CSB-MP398088BPF
CSB-EP398088BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. SsrA-binding protein (smpB) CSB-YP398089BPF
CSB-EP398089BPF
CSB-BP398089BPF
CSB-MP398089BPF
CSB-EP398089BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Formyl-coenzyme A transferase (frc) CSB-YP398090BPF
CSB-EP398090BPF
CSB-BP398090BPF
CSB-MP398090BPF
CSB-EP398090BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Bifunctional protein HldE (hldE) CSB-YP398091BPF
CSB-EP398091BPF
CSB-BP398091BPF
CSB-MP398091BPF
CSB-EP398091BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Ketol-acid reductoisomerase (ilvC) CSB-YP398092BPF
CSB-EP398092BPF
CSB-BP398092BPF
CSB-MP398092BPF
CSB-EP398092BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP398093BPF
CSB-EP398093BPF
CSB-BP398093BPF
CSB-MP398093BPF
CSB-EP398093BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP398094BPF
CSB-EP398094BPF
CSB-BP398094BPF
CSB-MP398094BPF
CSB-EP398094BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 60 kDa chaperonin 3 (groL3), partial CSB-YP398095BPF
CSB-EP398095BPF
CSB-BP398095BPF
CSB-MP398095BPF
CSB-EP398095BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Ribonuclease H (rnhA) CSB-YP398096BPF
CSB-EP398096BPF
CSB-BP398096BPF
CSB-MP398096BPF
CSB-EP398096BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Cytochrome c oxidase assembly protein CtaG (ctaG), partial CSB-YP398097BPF
CSB-EP398097BPF
CSB-BP398097BPF
CSB-MP398097BPF
CSB-EP398097BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A RNA-directed RNA polymerase, partial CSB-YP398098ROC
CSB-EP398098ROC
CSB-BP398098ROC
CSB-MP398098ROC
CSB-EP398098ROC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brassica oleracea var. italica Homocysteine S-methyltransferase 1 (HMT1) CSB-YP398099BPG
CSB-EP398099BPG
CSB-BP398099BPG
CSB-MP398099BPG
CSB-EP398099BPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus bottle-shaped virus Uncharacterized protein ORF91b (ORF91b) CSB-YP398100ATY
CSB-EP398100ATY
CSB-BP398100ATY
CSB-MP398100ATY
CSB-EP398100ATY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus bottle-shaped virus Uncharacterized protein ORF61c (ORF61c) CSB-YP398101ATY
CSB-EP398101ATY
CSB-BP398101ATY
CSB-MP398101ATY
CSB-EP398101ATY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus bottle-shaped virus Uncharacterized protein ORF61b (ORF61b) CSB-YP398102ATY
CSB-EP398102ATY
CSB-BP398102ATY
CSB-MP398102ATY
CSB-EP398102ATY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus bottle-shaped virus Uncharacterized protein ORF103 (ORF103), partial CSB-YP398103ATY1
CSB-EP398103ATY1
CSB-BP398103ATY1
CSB-MP398103ATY1
CSB-EP398103ATY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus bottle-shaped virus Putative transmembrane protein ORF346 (ORF346), partial CSB-YP398104ATY1
CSB-EP398104ATY1
CSB-BP398104ATY1
CSB-MP398104ATY1
CSB-EP398104ATY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus bottle-shaped virus Uncharacterized protein ORF107a (ORF107a) CSB-YP398105ATY
CSB-EP398105ATY
CSB-BP398105ATY
CSB-MP398105ATY
CSB-EP398105ATY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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