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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii 50S ribosomal protein L16 (rplP) CSB-YP398466VDY
CSB-EP398466VDY
CSB-BP398466VDY
CSB-MP398466VDY
CSB-EP398466VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii 30S ribosomal protein S14 (rpsN) CSB-YP398467VDY
CSB-EP398467VDY
CSB-BP398467VDY
CSB-MP398467VDY
CSB-EP398467VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii 50S ribosomal protein L30 (rpmD) CSB-YP398468VDY
CSB-EP398468VDY
CSB-BP398468VDY
CSB-MP398468VDY
CSB-EP398468VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP398469VDY
CSB-EP398469VDY
CSB-BP398469VDY
CSB-MP398469VDY
CSB-EP398469VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 50S ribosomal protein L31 (rpmE) CSB-YP398470PYG
CSB-EP398470PYG
CSB-BP398470PYG
CSB-MP398470PYG
CSB-EP398470PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP398471PYG
CSB-EP398471PYG
CSB-BP398471PYG
CSB-MP398471PYG
CSB-EP398471PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum DNA ligase (ligA), partial CSB-YP398472PYG
CSB-EP398472PYG
CSB-BP398472PYG
CSB-MP398472PYG
CSB-EP398472PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Protein MraZ (mraZ) CSB-YP398473PYG
CSB-EP398473PYG
CSB-BP398473PYG
CSB-MP398473PYG
CSB-EP398473PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Diaminopimelate epimerase (dapF) CSB-YP398474PYG
CSB-EP398474PYG
CSB-BP398474PYG
CSB-MP398474PYG
CSB-EP398474PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 30S ribosomal protein S16 (rpsP) CSB-YP398475PYG
CSB-EP398475PYG
CSB-BP398475PYG
CSB-MP398475PYG
CSB-EP398475PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 50S ribosomal protein L19 (rplS) CSB-YP398476PYG
CSB-EP398476PYG
CSB-BP398476PYG
CSB-MP398476PYG
CSB-EP398476PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum ATP-dependent helicase/deoxyribonuclease subunit B (addB), partial CSB-YP398477PYG
CSB-EP398477PYG
CSB-BP398477PYG
CSB-MP398477PYG
CSB-EP398477PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Cytidylate kinase (cmk) CSB-YP398478PYG
CSB-EP398478PYG
CSB-BP398478PYG
CSB-MP398478PYG
CSB-EP398478PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP398479PYG
CSB-EP398479PYG
CSB-BP398479PYG
CSB-MP398479PYG
CSB-EP398479PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Dihydrodipicolinate synthase (dapA) CSB-YP398480PYG
CSB-EP398480PYG
CSB-BP398480PYG
CSB-MP398480PYG
CSB-EP398480PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum tRNA pseudouridine synthase B (truB) CSB-YP398481PYG
CSB-EP398481PYG
CSB-BP398481PYG
CSB-MP398481PYG
CSB-EP398481PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum N utilization substance protein B homolog (nusB) CSB-YP398482PYG
CSB-EP398482PYG
CSB-BP398482PYG
CSB-MP398482PYG
CSB-EP398482PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Chorismate synthase (aroC) CSB-YP398483PYG
CSB-EP398483PYG
CSB-BP398483PYG
CSB-MP398483PYG
CSB-EP398483PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Aspartate--tRNA ligase (aspS), partial CSB-YP398484PYG
CSB-EP398484PYG
CSB-BP398484PYG
CSB-MP398484PYG
CSB-EP398484PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Probable transcriptional regulatory protein PTH_1024 (PTH_1024) CSB-YP398485PYG
CSB-EP398485PYG
CSB-BP398485PYG
CSB-MP398485PYG
CSB-EP398485PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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