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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Vibrio cholerae serotype O1 Type 4 prepilin-like proteins leader peptide-processing enzyme (tcpJ), partial CSB-YP399067VEY1
CSB-EP399067VEY1
CSB-BP399067VEY1
CSB-MP399067VEY1
CSB-EP399067VEY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 D-amino acid dehydrogenase small subunit (dadA) CSB-YP399068VEY
CSB-EP399068VEY
CSB-BP399068VEY
CSB-MP399068VEY
CSB-EP399068VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Vibriobactin-specific isochorismatase (vibB) CSB-YP399069VEY
CSB-EP399069VEY
CSB-BP399069VEY
CSB-MP399069VEY
CSB-EP399069VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Histidine--tRNA ligase (hisS) CSB-YP399070VEY
CSB-EP399070VEY
CSB-BP399070VEY
CSB-MP399070VEY
CSB-EP399070VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 50S ribosomal protein L9 (rplI) CSB-YP399071VEY
CSB-EP399071VEY
CSB-BP399071VEY
CSB-MP399071VEY
CSB-EP399071VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 50S ribosomal protein L10 (rplJ) CSB-YP399072VEY
CSB-EP399072VEY
CSB-BP399072VEY
CSB-MP399072VEY
CSB-EP399072VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP399073VEY
CSB-EP399073VEY
CSB-BP399073VEY
CSB-MP399073VEY
CSB-EP399073VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 50S ribosomal protein L28 (rpmB) CSB-YP399074VEY
CSB-EP399074VEY
CSB-BP399074VEY
CSB-MP399074VEY
CSB-EP399074VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Phosphopantetheine adenylyltransferase (coaD) CSB-YP399075VEY
CSB-EP399075VEY
CSB-BP399075VEY
CSB-MP399075VEY
CSB-EP399075VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Sulfurtransferase TusA homolog (tusA) CSB-YP399076VEY
CSB-EP399076VEY
CSB-BP399076VEY
CSB-MP399076VEY
CSB-EP399076VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Protein smg homolog (smg) CSB-YP399077VEY
CSB-EP399077VEY
CSB-BP399077VEY
CSB-MP399077VEY
CSB-EP399077VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP399078VEY1
CSB-EP399078VEY1
CSB-BP399078VEY1
CSB-MP399078VEY1
CSB-EP399078VEY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Tyrosine recombinase XerC (xerC) CSB-YP399079VEY
CSB-EP399079VEY
CSB-BP399079VEY
CSB-MP399079VEY
CSB-EP399079VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Glutamate racemase (murI) CSB-YP399080VEY
CSB-EP399080VEY
CSB-BP399080VEY
CSB-MP399080VEY
CSB-EP399080VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Elongation factor P (efp) CSB-YP399081VEY
CSB-EP399081VEY
CSB-BP399081VEY
CSB-MP399081VEY
CSB-EP399081VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Protein-export protein secB (secB) CSB-YP399082VEY
CSB-EP399082VEY
CSB-BP399082VEY
CSB-MP399082VEY
CSB-EP399082VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP399083VEY
CSB-EP399083VEY
CSB-BP399083VEY
CSB-MP399083VEY
CSB-EP399083VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 50S ribosomal protein L16 (rplP) CSB-YP399084VEY
CSB-EP399084VEY
CSB-BP399084VEY
CSB-MP399084VEY
CSB-EP399084VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 30S ribosomal protein S19 (rpsS) CSB-YP399085VEY
CSB-EP399085VEY
CSB-BP399085VEY
CSB-MP399085VEY
CSB-EP399085VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 50S ribosomal protein L3 (rplC) CSB-YP399086VEY
CSB-EP399086VEY
CSB-BP399086VEY
CSB-MP399086VEY
CSB-EP399086VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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