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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio cholerae serotype O1 UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP398666VEY
CSB-EP398666VEY
CSB-BP398666VEY
CSB-MP398666VEY
CSB-EP398666VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Ribosomal protein L11 methyltransferase (prmA) CSB-YP398667VEY
CSB-EP398667VEY
CSB-BP398667VEY
CSB-MP398667VEY
CSB-EP398667VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP398668VEY
CSB-EP398668VEY
CSB-BP398668VEY
CSB-MP398668VEY
CSB-EP398668VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 tRNA modification GTPase MnmE (mnmE) CSB-YP398669VEY
CSB-EP398669VEY
CSB-BP398669VEY
CSB-MP398669VEY
CSB-EP398669VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP398670VEY
CSB-EP398670VEY
CSB-BP398670VEY
CSB-MP398670VEY
CSB-EP398670VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Protein CyaY (cyaY) CSB-YP398671VEY
CSB-EP398671VEY
CSB-BP398671VEY
CSB-MP398671VEY
CSB-EP398671VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Vitamin B12 transporter BtuB (btuB), partial CSB-YP398672VEY
CSB-EP398672VEY
CSB-BP398672VEY
CSB-MP398672VEY
CSB-EP398672VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 50S ribosomal protein L31 (rpmE) CSB-YP398673VEY
CSB-EP398673VEY
CSB-BP398673VEY
CSB-MP398673VEY
CSB-EP398673VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP398674VEY
CSB-EP398674VEY
CSB-BP398674VEY
CSB-MP398674VEY
CSB-EP398674VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Acetylornithine deacetylase (argE) CSB-YP398675VEY
CSB-EP398675VEY
CSB-BP398675VEY
CSB-MP398675VEY
CSB-EP398675VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Adenylosuccinate synthetase (purA) CSB-YP398676VEY
CSB-EP398676VEY
CSB-BP398676VEY
CSB-MP398676VEY
CSB-EP398676VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 50S ribosomal protein L22 (rplV) CSB-YP398677VEY
CSB-EP398677VEY
CSB-BP398677VEY
CSB-MP398677VEY
CSB-EP398677VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 50S ribosomal protein L24 (rplX) CSB-YP398678VEY
CSB-EP398678VEY
CSB-BP398678VEY
CSB-MP398678VEY
CSB-EP398678VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP398679VEY
CSB-EP398679VEY
CSB-BP398679VEY
CSB-MP398679VEY
CSB-EP398679VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP398680VEY
CSB-EP398680VEY
CSB-BP398680VEY
CSB-MP398680VEY
CSB-EP398680VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP398681VEY
CSB-EP398681VEY
CSB-BP398681VEY
CSB-MP398681VEY
CSB-EP398681VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP398682VEY
CSB-EP398682VEY
CSB-BP398682VEY
CSB-MP398682VEY
CSB-EP398682VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Bifunctional protein HldE (hldE) CSB-YP398683VEY
CSB-EP398683VEY
CSB-BP398683VEY
CSB-MP398683VEY
CSB-EP398683VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Dihydrodipicolinate reductase (dapB) CSB-YP398684VEY
CSB-EP398684VEY
CSB-BP398684VEY
CSB-MP398684VEY
CSB-EP398684VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Sigma factor-binding protein crl (crl) CSB-YP398685VEY
CSB-EP398685VEY
CSB-BP398685VEY
CSB-MP398685VEY
CSB-EP398685VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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