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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechococcus sp. 50S ribosomal protein L29 (rpmC) CSB-YP400827SVC
CSB-EP400827SVC
CSB-BP400827SVC
CSB-MP400827SVC
CSB-EP400827SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L4 (rplD) CSB-YP400828SVC
CSB-EP400828SVC
CSB-BP400828SVC
CSB-MP400828SVC
CSB-EP400828SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Bifunctional pantoate ligase/cytidylate kinase (panC/cmk) CSB-YP400829SVC
CSB-EP400829SVC
CSB-BP400829SVC
CSB-MP400829SVC
CSB-EP400829SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Cytochrome b6 (petB), partial CSB-YP400830SVC1
CSB-EP400830SVC1
CSB-BP400830SVC1
CSB-MP400830SVC1
CSB-EP400830SVC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. GTPase Der (der) CSB-YP400831SVC
CSB-EP400831SVC
CSB-BP400831SVC
CSB-MP400831SVC
CSB-EP400831SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem I reaction center subunit XII (psaM), partial CSB-YP400832SVC
CSB-EP400832SVC
CSB-BP400832SVC
CSB-MP400832SVC
CSB-EP400832SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Chromosomal replication initiator protein DnaA (dnaA) CSB-YP400833SVC
CSB-EP400833SVC
CSB-BP400833SVC
CSB-MP400833SVC
CSB-EP400833SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem Q (B) protein 2, partial CSB-YP400834SVC1
CSB-EP400834SVC1
CSB-BP400834SVC1
CSB-MP400834SVC1
CSB-EP400834SVC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Anthranilate phosphoribosyltransferase (trpD) CSB-YP400835SVC
CSB-EP400835SVC
CSB-BP400835SVC
CSB-MP400835SVC
CSB-EP400835SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L32 (rpmF) CSB-YP400836SVC
CSB-EP400836SVC
CSB-BP400836SVC
CSB-MP400836SVC
CSB-EP400836SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP400837SVC
CSB-EP400837SVC
CSB-BP400837SVC
CSB-MP400837SVC
CSB-EP400837SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Cytochrome c biogenesis protein CcsA (ccsA), partial CSB-YP400838SVC1
CSB-EP400838SVC1
CSB-BP400838SVC1
CSB-MP400838SVC1
CSB-EP400838SVC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP400839SVC
CSB-EP400839SVC
CSB-BP400839SVC
CSB-MP400839SVC
CSB-EP400839SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Biotin synthase (bioB) CSB-YP400840SVC
CSB-EP400840SVC
CSB-BP400840SVC
CSB-MP400840SVC
CSB-EP400840SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP400841SVC
CSB-EP400841SVC
CSB-BP400841SVC
CSB-MP400841SVC
CSB-EP400841SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP400842SVC
CSB-EP400842SVC
CSB-BP400842SVC
CSB-MP400842SVC
CSB-EP400842SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP400843SVC
CSB-EP400843SVC
CSB-BP400843SVC
CSB-MP400843SVC
CSB-EP400843SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Cytochrome c biogenesis protein CcsB (ccsB), partial CSB-YP400844SVC1
CSB-EP400844SVC1
CSB-BP400844SVC1
CSB-MP400844SVC1
CSB-EP400844SVC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribose-5-phosphate isomerase A (rpiA) CSB-YP400845SVC
CSB-EP400845SVC
CSB-BP400845SVC
CSB-MP400845SVC
CSB-EP400845SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Undecaprenyl-diphosphatase (uppP), partial CSB-YP400846SVC1
CSB-EP400846SVC1
CSB-BP400846SVC1
CSB-MP400846SVC1
CSB-EP400846SVC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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