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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus 30S ribosomal protein S14 type Z (rpsZ) CSB-YP401027SUJ
CSB-EP401027SUJ
CSB-BP401027SUJ
CSB-MP401027SUJ
CSB-EP401027SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L16 (rplP) CSB-YP401028SUJ
CSB-EP401028SUJ
CSB-BP401028SUJ
CSB-MP401028SUJ
CSB-EP401028SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP401029SUJ
CSB-EP401029SUJ
CSB-BP401029SUJ
CSB-MP401029SUJ
CSB-EP401029SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Formimidoylglutamase (hutG) CSB-YP401030SUJ
CSB-EP401030SUJ
CSB-BP401030SUJ
CSB-MP401030SUJ
CSB-EP401030SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable nitrate transporter narT (narT), partial CSB-YP401031SUJ
CSB-EP401031SUJ
CSB-BP401031SUJ
CSB-MP401031SUJ
CSB-EP401031SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae Pseudouridine-5'-phosphate glycosidase (psuG) CSB-YP401032MWC
CSB-EP401032MWC
CSB-BP401032MWC
CSB-MP401032MWC
CSB-EP401032MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae 50S ribosomal protein L1 (rplA) CSB-YP401033MWC
CSB-EP401033MWC
CSB-BP401033MWC
CSB-MP401033MWC
CSB-EP401033MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae Chaperone protein DnaK (dnaK), partial CSB-YP401034MWC
CSB-EP401034MWC
CSB-BP401034MWC
CSB-MP401034MWC
CSB-EP401034MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP401035MWC
CSB-EP401035MWC
CSB-BP401035MWC
CSB-MP401035MWC
CSB-EP401035MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP401036MWC1
CSB-EP401036MWC1
CSB-BP401036MWC1
CSB-MP401036MWC1
CSB-EP401036MWC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae Alanine--tRNA ligase (alaS), partial CSB-YP401037MWC
CSB-EP401037MWC
CSB-BP401037MWC
CSB-MP401037MWC
CSB-EP401037MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae Probable endonuclease 4 (nfo) CSB-YP401038MWC
CSB-EP401038MWC
CSB-BP401038MWC
CSB-MP401038MWC
CSB-EP401038MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae 50S ribosomal protein L14 (rplN) CSB-YP401039MWC
CSB-EP401039MWC
CSB-BP401039MWC
CSB-MP401039MWC
CSB-EP401039MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae Glutamate--tRNA ligase (gltX) CSB-YP401040MWC
CSB-EP401040MWC
CSB-BP401040MWC
CSB-MP401040MWC
CSB-EP401040MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae Probable transcriptional regulatory protein MAG6590 (MAG6590) CSB-YP401041MWC
CSB-EP401041MWC
CSB-BP401041MWC
CSB-MP401041MWC
CSB-EP401041MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae Cytidylate kinase (cmk) CSB-YP401042MWC
CSB-EP401042MWC
CSB-BP401042MWC
CSB-MP401042MWC
CSB-EP401042MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumis sativus Putative uncharacterized protein ycf15 (ycf15) CSB-YP401043CXP
CSB-EP401043CXP
CSB-BP401043CXP
CSB-MP401043CXP
CSB-EP401043CXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. 4-chlorobenzoate--CoA ligase, partial CSB-YP401044PZO
CSB-EP401044PZO
CSB-BP401044PZO
CSB-MP401044PZO
CSB-EP401044PZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium vivax tRNA (guanine (37)-N1)-methyltransferase (PVX_099140), partial CSB-YP401045EWW
CSB-EP401045EWW
CSB-BP401045EWW
CSB-MP401045EWW
CSB-EP401045EWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fascicularis UPF0731 protein C6orf225 homolog (QtsA-19618) CSB-YP401046MOV
CSB-EP401046MOV
CSB-BP401046MOV
CSB-MP401046MOV
CSB-EP401046MOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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