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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Dichelobacter nodosus 30S ribosomal protein S16 (rpsP) CSB-YP399907DHZ
CSB-EP399907DHZ
CSB-BP399907DHZ
CSB-MP399907DHZ
CSB-EP399907DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP399908DHZ
CSB-EP399908DHZ
CSB-BP399908DHZ
CSB-MP399908DHZ
CSB-EP399908DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus 50S ribosomal protein L34 (rpmH) CSB-YP399909DHZ
CSB-EP399909DHZ
CSB-BP399909DHZ
CSB-MP399909DHZ
CSB-EP399909DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Lysine--tRNA ligase (lysS) CSB-YP399910DHZ
CSB-EP399910DHZ
CSB-BP399910DHZ
CSB-MP399910DHZ
CSB-EP399910DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Probable septum site-determining protein MinC (minC) CSB-YP399911DHZ
CSB-EP399911DHZ
CSB-BP399911DHZ
CSB-MP399911DHZ
CSB-EP399911DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Chaperone protein DnaK (dnaK), partial CSB-YP399912DHZ
CSB-EP399912DHZ
CSB-BP399912DHZ
CSB-MP399912DHZ
CSB-EP399912DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 50S ribosomal protein L35 (rpmI) CSB-YP399913VEY
CSB-EP399913VEY
CSB-BP399913VEY
CSB-MP399913VEY
CSB-EP399913VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 UPF0271 protein VC0395_0716/VC395_A0539 (VC0395_0716, VC395_A0539) CSB-YP399914VEY
CSB-EP399914VEY
CSB-BP399914VEY
CSB-MP399914VEY
CSB-EP399914VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Cobyric acid synthase (cobQ) CSB-YP399915VEY
CSB-EP399915VEY
CSB-BP399915VEY
CSB-MP399915VEY
CSB-EP399915VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 D-alanine--D-alanine ligase (ddl) CSB-YP399916VEY
CSB-EP399916VEY
CSB-BP399916VEY
CSB-MP399916VEY
CSB-EP399916VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 GlcNAc-binding protein A (gbpA) CSB-YP399917VEY
CSB-EP399917VEY
CSB-BP399917VEY
CSB-MP399917VEY
CSB-EP399917VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Putative pterin-4-alpha-carbinolamine dehydratase (phhB) CSB-YP399918VEY
CSB-EP399918VEY
CSB-BP399918VEY
CSB-MP399918VEY
CSB-EP399918VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP399919VEY
CSB-EP399919VEY
CSB-BP399919VEY
CSB-MP399919VEY
CSB-EP399919VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Probable transcriptional regulatory protein VC0395_0124/VC395_A0006 (VC0395_0124, VC395_A0006) CSB-YP399920VEY
CSB-EP399920VEY
CSB-BP399920VEY
CSB-MP399920VEY
CSB-EP399920VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP399921VEY
CSB-EP399921VEY
CSB-BP399921VEY
CSB-MP399921VEY
CSB-EP399921VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Integration host factor subunit alpha (ihfA) CSB-YP399922VEY
CSB-EP399922VEY
CSB-BP399922VEY
CSB-MP399922VEY
CSB-EP399922VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Imidazolonepropionase (hutI) CSB-YP399923VEY
CSB-EP399923VEY
CSB-BP399923VEY
CSB-MP399923VEY
CSB-EP399923VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Aspartate--tRNA ligase (aspS), partial CSB-YP399924VEY
CSB-EP399924VEY
CSB-BP399924VEY
CSB-MP399924VEY
CSB-EP399924VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 High frequency lysogenization protein HflD homolog (hflD) CSB-YP399925VEY
CSB-EP399925VEY
CSB-BP399925VEY
CSB-MP399925VEY
CSB-EP399925VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 UPF0260 protein VC0395_A0576/VC395_1072 (VC0395_A0576, VC395_1072) CSB-YP399926VEY
CSB-EP399926VEY
CSB-BP399926VEY
CSB-MP399926VEY
CSB-EP399926VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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