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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Vibrio cholerae serotype O1 Protein CrcB homolog (crcB), partial CSB-YP399947VEY1
CSB-EP399947VEY1
CSB-BP399947VEY1
CSB-MP399947VEY1
CSB-EP399947VEY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 LexA repressor (lexA) CSB-YP399948VEY
CSB-EP399948VEY
CSB-BP399948VEY
CSB-MP399948VEY
CSB-EP399948VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP399949VEY
CSB-EP399949VEY
CSB-BP399949VEY
CSB-MP399949VEY
CSB-EP399949VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Soluble pyridine nucleotide transhydrogenase (sthA) CSB-YP399950VEY
CSB-EP399950VEY
CSB-BP399950VEY
CSB-MP399950VEY
CSB-EP399950VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 UPF0761 membrane protein VC0395_A2314/VC395_2854 (VC0395_A2314, VC395_2854), partial CSB-YP399951VEY1
CSB-EP399951VEY1
CSB-BP399951VEY1
CSB-MP399951VEY1
CSB-EP399951VEY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Cell division protein ZapB (zapB) CSB-YP399952VEY
CSB-EP399952VEY
CSB-BP399952VEY
CSB-MP399952VEY
CSB-EP399952VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Fumarate reductase subunit C (frdC), partial CSB-YP399953VEY1
CSB-EP399953VEY1
CSB-BP399953VEY1
CSB-MP399953VEY1
CSB-EP399953VEY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP399954VEY
CSB-EP399954VEY
CSB-BP399954VEY
CSB-MP399954VEY
CSB-EP399954VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Shikimate kinase (aroK) CSB-YP399955VEY
CSB-EP399955VEY
CSB-BP399955VEY
CSB-MP399955VEY
CSB-EP399955VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 50S ribosomal protein L2 (rplB) CSB-YP399956VEY
CSB-EP399956VEY
CSB-BP399956VEY
CSB-MP399956VEY
CSB-EP399956VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 30S ribosomal protein S14 (rpsN) CSB-YP399957VEY
CSB-EP399957VEY
CSB-BP399957VEY
CSB-MP399957VEY
CSB-EP399957VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 30S ribosomal protein S5 (rpsE) CSB-YP399958VEY
CSB-EP399958VEY
CSB-BP399958VEY
CSB-MP399958VEY
CSB-EP399958VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP399959VEY
CSB-EP399959VEY
CSB-BP399959VEY
CSB-MP399959VEY
CSB-EP399959VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP399960VEY
CSB-EP399960VEY
CSB-BP399960VEY
CSB-MP399960VEY
CSB-EP399960VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 tRNA-modifying protein ygfZ (VC0395_A2049, VC395_2587) CSB-YP399961VEY
CSB-EP399961VEY
CSB-BP399961VEY
CSB-MP399961VEY
CSB-EP399961VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP399962VEY
CSB-EP399962VEY
CSB-BP399962VEY
CSB-MP399962VEY
CSB-EP399962VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Enolase (eno) CSB-YP399963VEY
CSB-EP399963VEY
CSB-BP399963VEY
CSB-MP399963VEY
CSB-EP399963VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP399964VEY
CSB-EP399964VEY
CSB-BP399964VEY
CSB-MP399964VEY
CSB-EP399964VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Protein translocase subunit SecA (secA), partial CSB-YP399965VEY
CSB-EP399965VEY
CSB-BP399965VEY
CSB-MP399965VEY
CSB-EP399965VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 UPF0246 protein VC0395_A1934/VC395_2470 (VC0395_A1934, VC395_2470) CSB-YP399966VEY
CSB-EP399966VEY
CSB-BP399966VEY
CSB-MP399966VEY
CSB-EP399966VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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