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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechococcus sp. Lysine--tRNA ligase (lysS) CSB-YP400107SVC
CSB-EP400107SVC
CSB-BP400107SVC
CSB-MP400107SVC
CSB-EP400107SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribosome maturation factor RimM (rimM) CSB-YP400108SVC
CSB-EP400108SVC
CSB-BP400108SVC
CSB-MP400108SVC
CSB-EP400108SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Cytochrome b559 subunit alpha (psbE), partial CSB-YP400109SVC1
CSB-EP400109SVC1
CSB-BP400109SVC1
CSB-MP400109SVC1
CSB-EP400109SVC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP400110SVC
CSB-EP400110SVC
CSB-BP400110SVC
CSB-MP400110SVC
CSB-EP400110SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP400111SVC
CSB-EP400111SVC
CSB-BP400111SVC
CSB-MP400111SVC
CSB-EP400111SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP400112SVC
CSB-EP400112SVC
CSB-BP400112SVC
CSB-MP400112SVC
CSB-EP400112SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L31 (rpmE) CSB-YP400113SVC
CSB-EP400113SVC
CSB-BP400113SVC
CSB-MP400113SVC
CSB-EP400113SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L36 (rpmJ) CSB-YP400114SVC
CSB-EP400114SVC
CSB-BP400114SVC
CSB-MP400114SVC
CSB-EP400114SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L6 (rplF) CSB-YP400115SVC
CSB-EP400115SVC
CSB-BP400115SVC
CSB-MP400115SVC
CSB-EP400115SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S3 (rpsC) CSB-YP400116SVC
CSB-EP400116SVC
CSB-BP400116SVC
CSB-MP400116SVC
CSB-EP400116SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L3 (rplC) CSB-YP400117SVC
CSB-EP400117SVC
CSB-BP400117SVC
CSB-MP400117SVC
CSB-EP400117SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. S-adenosylmethionine synthase (metK) CSB-YP400118SVC
CSB-EP400118SVC
CSB-BP400118SVC
CSB-MP400118SVC
CSB-EP400118SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 3-dehydroquinate dehydratase (aroQ) CSB-YP400119SVC
CSB-EP400119SVC
CSB-BP400119SVC
CSB-MP400119SVC
CSB-EP400119SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S4 (rpsD) CSB-YP400120SVC
CSB-EP400120SVC
CSB-BP400120SVC
CSB-MP400120SVC
CSB-EP400120SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Glycogen synthase (glgA), partial CSB-YP400121SVC
CSB-EP400121SVC
CSB-BP400121SVC
CSB-MP400121SVC
CSB-EP400121SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP400122SVC
CSB-EP400122SVC
CSB-BP400122SVC
CSB-MP400122SVC
CSB-EP400122SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phosphopantetheine adenylyltransferase (coaD) CSB-YP400123SVC
CSB-EP400123SVC
CSB-BP400123SVC
CSB-MP400123SVC
CSB-EP400123SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Glutamyl-tRNA reductase (hemA) CSB-YP400124SVC
CSB-EP400124SVC
CSB-BP400124SVC
CSB-MP400124SVC
CSB-EP400124SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 33 kDa chaperonin (hslO) CSB-YP400125SVC
CSB-EP400125SVC
CSB-BP400125SVC
CSB-MP400125SVC
CSB-EP400125SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Cysteine--tRNA ligase (cysS) CSB-YP400126SVC
CSB-EP400126SVC
CSB-BP400126SVC
CSB-MP400126SVC
CSB-EP400126SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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