Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Dehalococcoides sp. Transcriptional repressor NrdR (nrdR) CSB-YP400047DHU
CSB-EP400047DHU
CSB-BP400047DHU
CSB-MP400047DHU
CSB-EP400047DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Cytidylate kinase (cmk) CSB-YP400048ATZ
CSB-EP400048ATZ
CSB-BP400048ATZ
CSB-MP400048ATZ
CSB-EP400048ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Probable potassium transport system protein kup (kup), partial CSB-YP400049ATZ
CSB-EP400049ATZ
CSB-BP400049ATZ
CSB-MP400049ATZ
CSB-EP400049ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Bifunctional protein HldE (hldE) CSB-YP400050ATZ
CSB-EP400050ATZ
CSB-BP400050ATZ
CSB-MP400050ATZ
CSB-EP400050ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum 30S ribosomal protein S4 (rpsD) CSB-YP400051ATZ
CSB-EP400051ATZ
CSB-BP400051ATZ
CSB-MP400051ATZ
CSB-EP400051ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Putative pterin-4-alpha-carbinolamine dehydratase (Acry_0770) CSB-YP400052ATZ
CSB-EP400052ATZ
CSB-BP400052ATZ
CSB-MP400052ATZ
CSB-EP400052ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Putative Holliday junction resolvase (Acry_0873) CSB-YP400053ATZ
CSB-EP400053ATZ
CSB-BP400053ATZ
CSB-MP400053ATZ
CSB-EP400053ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP400054ATZ
CSB-EP400054ATZ
CSB-BP400054ATZ
CSB-MP400054ATZ
CSB-EP400054ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum NADH-quinone oxidoreductase subunit N 2 (nuoN2), partial CSB-YP400055ATZ
CSB-EP400055ATZ
CSB-BP400055ATZ
CSB-MP400055ATZ
CSB-EP400055ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Heme A synthase 1 (ctaA1), partial CSB-YP400056ATZ
CSB-EP400056ATZ
CSB-BP400056ATZ
CSB-MP400056ATZ
CSB-EP400056ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP400057ATZ
CSB-EP400057ATZ
CSB-BP400057ATZ
CSB-MP400057ATZ
CSB-EP400057ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Homoserine kinase (thrB) CSB-YP400058ATZ
CSB-EP400058ATZ
CSB-BP400058ATZ
CSB-MP400058ATZ
CSB-EP400058ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Elongation factor P (efp) CSB-YP400059ATZ
CSB-EP400059ATZ
CSB-BP400059ATZ
CSB-MP400059ATZ
CSB-EP400059ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Imidazolonepropionase (hutI) CSB-YP400060ATZ
CSB-EP400060ATZ
CSB-BP400060ATZ
CSB-MP400060ATZ
CSB-EP400060ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Hydroxylamine reductase (hcp), partial CSB-YP400061ATZ
CSB-EP400061ATZ
CSB-BP400061ATZ
CSB-MP400061ATZ
CSB-EP400061ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum 50S ribosomal protein L17 (rplQ) CSB-YP400062ATZ
CSB-EP400062ATZ
CSB-BP400062ATZ
CSB-MP400062ATZ
CSB-EP400062ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum 50S ribosomal protein L30 (rpmD) CSB-YP400063ATZ
CSB-EP400063ATZ
CSB-BP400063ATZ
CSB-MP400063ATZ
CSB-EP400063ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum 50S ribosomal protein L29 (rpmC) CSB-YP400064ATZ
CSB-EP400064ATZ
CSB-BP400064ATZ
CSB-MP400064ATZ
CSB-EP400064ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum 50S ribosomal protein L3 (rplC) CSB-YP400065ATZ
CSB-EP400065ATZ
CSB-BP400065ATZ
CSB-MP400065ATZ
CSB-EP400065ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum 50S ribosomal protein L10 (rplJ) CSB-YP400066ATZ
CSB-EP400066ATZ
CSB-BP400066ATZ
CSB-MP400066ATZ
CSB-EP400066ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>