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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Geobacter uraniireducens Pantothenate synthetase (panC) CSB-YP401507GEI
CSB-EP401507GEI
CSB-BP401507GEI
CSB-MP401507GEI
CSB-EP401507GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP401508GEI
CSB-EP401508GEI
CSB-BP401508GEI
CSB-MP401508GEI
CSB-EP401508GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens UPF0345 protein Gura_2227 (Gura_2227) CSB-YP401509GEI
CSB-EP401509GEI
CSB-BP401509GEI
CSB-MP401509GEI
CSB-EP401509GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens DNA mismatch repair protein MutS (mutS), partial CSB-YP401510GEI
CSB-EP401510GEI
CSB-BP401510GEI
CSB-MP401510GEI
CSB-EP401510GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP401511GEI
CSB-EP401511GEI
CSB-BP401511GEI
CSB-MP401511GEI
CSB-EP401511GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Type III pantothenate kinase (coaX) CSB-YP401512GEI
CSB-EP401512GEI
CSB-BP401512GEI
CSB-MP401512GEI
CSB-EP401512GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP401513GEI
CSB-EP401513GEI
CSB-BP401513GEI
CSB-MP401513GEI
CSB-EP401513GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens GTPase Der (der) CSB-YP401514GEI
CSB-EP401514GEI
CSB-BP401514GEI
CSB-MP401514GEI
CSB-EP401514GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP401515GEI
CSB-EP401515GEI
CSB-BP401515GEI
CSB-MP401515GEI
CSB-EP401515GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Na (+)/H (+) antiporter nhaA, partial CSB-YP401516GEI
CSB-EP401516GEI
CSB-BP401516GEI
CSB-MP401516GEI
CSB-EP401516GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 30S ribosomal protein S18 (rpsR) CSB-YP401517GEI
CSB-EP401517GEI
CSB-BP401517GEI
CSB-MP401517GEI
CSB-EP401517GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 30S ribosomal protein S2 (rpsB) CSB-YP401518GEI
CSB-EP401518GEI
CSB-BP401518GEI
CSB-MP401518GEI
CSB-EP401518GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP401519GEI
CSB-EP401519GEI
CSB-BP401519GEI
CSB-MP401519GEI
CSB-EP401519GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens UPF0178 protein Gura_4014 (Gura_4014) CSB-YP401520GEI
CSB-EP401520GEI
CSB-BP401520GEI
CSB-MP401520GEI
CSB-EP401520GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP401521GEI
CSB-EP401521GEI
CSB-BP401521GEI
CSB-MP401521GEI
CSB-EP401521GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP401522GEI
CSB-EP401522GEI
CSB-BP401522GEI
CSB-MP401522GEI
CSB-EP401522GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP401523GEI
CSB-EP401523GEI
CSB-BP401523GEI
CSB-MP401523GEI
CSB-EP401523GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Putative phosphotransferase Gura_4292 (Gura_4292) CSB-YP401524GEI
CSB-EP401524GEI
CSB-BP401524GEI
CSB-MP401524GEI
CSB-EP401524GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP401525GEI
CSB-EP401525GEI
CSB-BP401525GEI
CSB-MP401525GEI
CSB-EP401525GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Probable transcriptional regulatory protein Gura_1416 (Gura_1416) CSB-YP401526GEI
CSB-EP401526GEI
CSB-BP401526GEI
CSB-MP401526GEI
CSB-EP401526GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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