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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Synechococcus sp. UPF0145 protein SynWH7803_1684 (SynWH7803_1684) CSB-YP400127SVC
CSB-EP400127SVC
CSB-BP400127SVC
CSB-MP400127SVC
CSB-EP400127SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. D-alanine--D-alanine ligase (ddl) CSB-YP400128SVC
CSB-EP400128SVC
CSB-BP400128SVC
CSB-MP400128SVC
CSB-EP400128SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP400129SVC
CSB-EP400129SVC
CSB-BP400129SVC
CSB-MP400129SVC
CSB-EP400129SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribonuclease P protein component (rnpA) CSB-YP400130SVC
CSB-EP400130SVC
CSB-BP400130SVC
CSB-MP400130SVC
CSB-EP400130SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. tRNA pseudouridine synthase B (truB) CSB-YP400131SVC
CSB-EP400131SVC
CSB-BP400131SVC
CSB-MP400131SVC
CSB-EP400131SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. ATP synthase epsilon chain (atpC) CSB-YP400132SVC
CSB-EP400132SVC
CSB-BP400132SVC
CSB-MP400132SVC
CSB-EP400132SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribosome maturation factor RimP (rimP) CSB-YP400133SVC
CSB-EP400133SVC
CSB-BP400133SVC
CSB-MP400133SVC
CSB-EP400133SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP400134SVC
CSB-EP400134SVC
CSB-BP400134SVC
CSB-MP400134SVC
CSB-EP400134SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. NAD (P)H-quinone oxidoreductase subunit O CSB-YP400135SVC
CSB-EP400135SVC
CSB-BP400135SVC
CSB-MP400135SVC
CSB-EP400135SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. NAD (P)H-quinone oxidoreductase chain 4, partial CSB-YP400136SVC
CSB-EP400136SVC
CSB-BP400136SVC
CSB-MP400136SVC
CSB-EP400136SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 1,4-dihydroxy-2-naphthoyl-CoA hydrolase (SynWH7803_2315) CSB-YP400137SVC
CSB-EP400137SVC
CSB-BP400137SVC
CSB-MP400137SVC
CSB-EP400137SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP400138SVC
CSB-EP400138SVC
CSB-BP400138SVC
CSB-MP400138SVC
CSB-EP400138SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L35 (rpmI) CSB-YP400139SVB
CSB-EP400139SVB
CSB-BP400139SVB
CSB-MP400139SVB
CSB-EP400139SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem II reaction center protein L (psbL), partial CSB-YP400140SVB
CSB-EP400140SVB
CSB-BP400140SVB
CSB-MP400140SVB
CSB-EP400140SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribosome-binding factor A (rbfA) CSB-YP400141SVB
CSB-EP400141SVB
CSB-BP400141SVB
CSB-MP400141SVB
CSB-EP400141SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem II reaction center X protein (psbX), partial CSB-YP400142SVB
CSB-EP400142SVB
CSB-BP400142SVB
CSB-MP400142SVB
CSB-EP400142SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem II reaction center protein M (psbM), partial CSB-YP400143SVB
CSB-EP400143SVB
CSB-BP400143SVB
CSB-MP400143SVB
CSB-EP400143SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 3-dehydroquinate dehydratase (aroQ) CSB-YP400144SVB
CSB-EP400144SVB
CSB-BP400144SVB
CSB-MP400144SVB
CSB-EP400144SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S14 (rpsN) CSB-YP400145SVB
CSB-EP400145SVB
CSB-BP400145SVB
CSB-MP400145SVB
CSB-EP400145SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Cytochrome b6-f complex iron-sulfur subunit (petC), partial CSB-YP400146SVB1
CSB-EP400146SVB1
CSB-BP400146SVB1
CSB-MP400146SVB1
CSB-EP400146SVB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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