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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus 50S ribosomal protein L22 (rplV) CSB-YP400307SUJ
CSB-EP400307SUJ
CSB-BP400307SUJ
CSB-MP400307SUJ
CSB-EP400307SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S10 (rpsJ) CSB-YP400308SUJ
CSB-EP400308SUJ
CSB-BP400308SUJ
CSB-MP400308SUJ
CSB-EP400308SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Urease subunit beta (ureB) CSB-YP400309SUJ
CSB-EP400309SUJ
CSB-BP400309SUJ
CSB-MP400309SUJ
CSB-EP400309SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Metallothiol transferase fosB (fosB) CSB-YP400310SUJ
CSB-EP400310SUJ
CSB-BP400310SUJ
CSB-MP400310SUJ
CSB-EP400310SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ferredoxin--NADP reductase (SaurJH9_2396) CSB-YP400311SUJ
CSB-EP400311SUJ
CSB-BP400311SUJ
CSB-MP400311SUJ
CSB-EP400311SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 6-carboxyhexanoate--CoA ligase (bioW) CSB-YP400312SUJ
CSB-EP400312SUJ
CSB-BP400312SUJ
CSB-MP400312SUJ
CSB-EP400312SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Copper chaperone CopZ (copZ) CSB-YP400313SUJ
CSB-EP400313SUJ
CSB-BP400313SUJ
CSB-MP400313SUJ
CSB-EP400313SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus HTH-type transcriptional regulator ArcR (arcR) CSB-YP400314SUJ
CSB-EP400314SUJ
CSB-BP400314SUJ
CSB-MP400314SUJ
CSB-EP400314SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae tRNA modification GTPase MnmE (mnmE) CSB-YP400315MWC
CSB-EP400315MWC
CSB-BP400315MWC
CSB-MP400315MWC
CSB-EP400315MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae 30S ribosomal protein S20 (rpsT) CSB-YP400316MWC
CSB-EP400316MWC
CSB-BP400316MWC
CSB-MP400316MWC
CSB-EP400316MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae Enolase (eno) CSB-YP400317MWC
CSB-EP400317MWC
CSB-BP400317MWC
CSB-MP400317MWC
CSB-EP400317MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae Putative Holliday junction resolvase (MAG4150) CSB-YP400318MWC
CSB-EP400318MWC
CSB-BP400318MWC
CSB-MP400318MWC
CSB-EP400318MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae Elongation factor 4 (lepA), partial CSB-YP400319MWC
CSB-EP400319MWC
CSB-BP400319MWC
CSB-MP400319MWC
CSB-EP400319MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae 30S ribosomal protein S14 type Z (rpsZ) CSB-YP400320MWC
CSB-EP400320MWC
CSB-BP400320MWC
CSB-MP400320MWC
CSB-EP400320MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae 50S ribosomal protein L22 (rplV) CSB-YP400321MWC
CSB-EP400321MWC
CSB-BP400321MWC
CSB-MP400321MWC
CSB-EP400321MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae 50S ribosomal protein L19 (rplS) CSB-YP400322MWC
CSB-EP400322MWC
CSB-BP400322MWC
CSB-MP400322MWC
CSB-EP400322MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae GTPase Der (der) CSB-YP400323MWC
CSB-EP400323MWC
CSB-BP400323MWC
CSB-MP400323MWC
CSB-EP400323MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumis sativus Translation initiation factor IF-1, chloroplastic (infA) CSB-YP400324CXP
CSB-EP400324CXP
CSB-BP400324CXP
CSB-MP400324CXP
CSB-EP400324CXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Medicago sativa subsp. varia Beta-xylosidase/alpha-L-arabinofuranosidase 1 (Xyl1), partial CSB-YP400325MNL
CSB-EP400325MNL
CSB-BP400325MNL
CSB-MP400325MNL
CSB-EP400325MNL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium vivax Translation factor GUF1 homolog, mitochondrial (PVX_099025) CSB-YP400326EWW
CSB-EP400326EWW
CSB-BP400326EWW
CSB-MP400326EWW
CSB-EP400326EWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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