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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Clostridium kluyveri Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP400347CWX
CSB-EP400347CWX
CSB-BP400347CWX
CSB-MP400347CWX
CSB-EP400347CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Tyrosine--tRNA ligase (tyrS) CSB-YP400348CWX
CSB-EP400348CWX
CSB-BP400348CWX
CSB-MP400348CWX
CSB-EP400348CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Maf-like protein CKL_0864 (CKL_0864) CSB-YP400349CWX
CSB-EP400349CWX
CSB-BP400349CWX
CSB-MP400349CWX
CSB-EP400349CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 50S ribosomal protein L21 (rplU) CSB-YP400350CWX
CSB-EP400350CWX
CSB-BP400350CWX
CSB-MP400350CWX
CSB-EP400350CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Chaperone protein DnaK (dnaK), partial CSB-YP400351CWX
CSB-EP400351CWX
CSB-BP400351CWX
CSB-MP400351CWX
CSB-EP400351CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri DNA repair protein recO (recO) CSB-YP400352CWX
CSB-EP400352CWX
CSB-BP400352CWX
CSB-MP400352CWX
CSB-EP400352CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Probable transaldolase (tal) CSB-YP400353CWX
CSB-EP400353CWX
CSB-BP400353CWX
CSB-MP400353CWX
CSB-EP400353CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Elongation factor P (efp) CSB-YP400354CWX
CSB-EP400354CWX
CSB-BP400354CWX
CSB-MP400354CWX
CSB-EP400354CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Indole-3-glycerol phosphate synthase (trpC) CSB-YP400355CWX
CSB-EP400355CWX
CSB-BP400355CWX
CSB-MP400355CWX
CSB-EP400355CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP400356CWX
CSB-EP400356CWX
CSB-BP400356CWX
CSB-MP400356CWX
CSB-EP400356CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri UPF0348 protein CKL_1389 (CKL_1389) CSB-YP400357CWX
CSB-EP400357CWX
CSB-BP400357CWX
CSB-MP400357CWX
CSB-EP400357CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri tRNA (guanine-N (1)-)-methyltransferase CSB-YP400358CWX
CSB-EP400358CWX
CSB-BP400358CWX
CSB-MP400358CWX
CSB-EP400358CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri tRNA pseudouridine synthase B (truB) CSB-YP400359CWX
CSB-EP400359CWX
CSB-BP400359CWX
CSB-MP400359CWX
CSB-EP400359CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Acetylglutamate kinase (argB) CSB-YP400360CWX
CSB-EP400360CWX
CSB-BP400360CWX
CSB-MP400360CWX
CSB-EP400360CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Phosphomethylpyrimidine synthase (thiC) CSB-YP400361CWX
CSB-EP400361CWX
CSB-BP400361CWX
CSB-MP400361CWX
CSB-EP400361CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At1g24485 (RWK1), partial CSB-YP400362DOA
CSB-EP400362DOA
CSB-BP400362DOA
CSB-MP400362DOA
CSB-EP400362DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Adenylate kinase isoenzyme 6 (TAF9) CSB-YP400363BO
CSB-EP400363BO
CSB-BP400363BO
CSB-MP400363BO
CSB-EP400363BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP400364FDT
CSB-EP400364FDT
CSB-BP400364FDT
CSB-MP400364FDT
CSB-EP400364FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP400365FDT
CSB-EP400365FDT
CSB-BP400365FDT
CSB-MP400365FDT
CSB-EP400365FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Ribosome-binding factor A (rbfA) CSB-YP400366FDT
CSB-EP400366FDT
CSB-BP400366FDT
CSB-MP400366FDT
CSB-EP400366FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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