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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Plasmodium vivax Elongation factor Ts, mitochondrial (PV1H14170c, PVX_119375) CSB-YP400327EWW
CSB-EP400327EWW
CSB-BP400327EWW
CSB-MP400327EWW
CSB-EP400327EWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP400328CWX
CSB-EP400328CWX
CSB-BP400328CWX
CSB-MP400328CWX
CSB-EP400328CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP400329CWX1
CSB-EP400329CWX1
CSB-BP400329CWX1
CSB-MP400329CWX1
CSB-EP400329CWX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Aspartate--tRNA ligase (aspS), partial CSB-YP400330CWX
CSB-EP400330CWX
CSB-BP400330CWX
CSB-MP400330CWX
CSB-EP400330CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP400331CWX
CSB-EP400331CWX
CSB-BP400331CWX
CSB-MP400331CWX
CSB-EP400331CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Enolase (eno) CSB-YP400332CWX
CSB-EP400332CWX
CSB-BP400332CWX
CSB-MP400332CWX
CSB-EP400332CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP400333CWX
CSB-EP400333CWX
CSB-BP400333CWX
CSB-MP400333CWX
CSB-EP400333CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Leucine--tRNA ligase (leuS), partial CSB-YP400334CWX
CSB-EP400334CWX
CSB-BP400334CWX
CSB-MP400334CWX
CSB-EP400334CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Isoleucine--tRNA ligase (ileS), partial CSB-YP400335CWX
CSB-EP400335CWX
CSB-BP400335CWX
CSB-MP400335CWX
CSB-EP400335CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 30S ribosomal protein S18 (rpsR) CSB-YP400336CWX
CSB-EP400336CWX
CSB-BP400336CWX
CSB-MP400336CWX
CSB-EP400336CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Serine--tRNA ligase (serS) CSB-YP400337CWX
CSB-EP400337CWX
CSB-BP400337CWX
CSB-MP400337CWX
CSB-EP400337CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP400338CWX
CSB-EP400338CWX
CSB-BP400338CWX
CSB-MP400338CWX
CSB-EP400338CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Foldase protein prsA (prsA) CSB-YP400339CWX
CSB-EP400339CWX
CSB-BP400339CWX
CSB-MP400339CWX
CSB-EP400339CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Putative ATP:guanido phosphotransferase CKL_0194 (CKL_0194) CSB-YP400340CWX
CSB-EP400340CWX
CSB-BP400340CWX
CSB-MP400340CWX
CSB-EP400340CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 50S ribosomal protein L33 (rpmG) CSB-YP400341CWX
CSB-EP400341CWX
CSB-BP400341CWX
CSB-MP400341CWX
CSB-EP400341CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 30S ribosomal protein S7 (rpsG) CSB-YP400342CWX
CSB-EP400342CWX
CSB-BP400342CWX
CSB-MP400342CWX
CSB-EP400342CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 50S ribosomal protein L2 (rplB) CSB-YP400343CWX
CSB-EP400343CWX
CSB-BP400343CWX
CSB-MP400343CWX
CSB-EP400343CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 50S ribosomal protein L5 (rplE) CSB-YP400344CWX
CSB-EP400344CWX
CSB-BP400344CWX
CSB-MP400344CWX
CSB-EP400344CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 50S ribosomal protein L15 (rplO) CSB-YP400345CWX
CSB-EP400345CWX
CSB-BP400345CWX
CSB-MP400345CWX
CSB-EP400345CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Redox-sensing transcriptional repressor rex (rex) CSB-YP400346CWX
CSB-EP400346CWX
CSB-BP400346CWX
CSB-MP400346CWX
CSB-EP400346CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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