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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Legionella pneumophila UPF0229 protein LPC_3097 (LPC_3097) CSB-YP400947LLJ
CSB-EP400947LLJ
CSB-BP400947LLJ
CSB-MP400947LLJ
CSB-EP400947LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Methionine--tRNA ligase (metG), partial CSB-YP400948LLJ
CSB-EP400948LLJ
CSB-BP400948LLJ
CSB-MP400948LLJ
CSB-EP400948LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP400949LLJ
CSB-EP400949LLJ
CSB-BP400949LLJ
CSB-MP400949LLJ
CSB-EP400949LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila ATP synthase gamma chain (atpG) CSB-YP400950LLJ
CSB-EP400950LLJ
CSB-BP400950LLJ
CSB-MP400950LLJ
CSB-EP400950LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Protein grpE (grpE) CSB-YP400951TKF
CSB-EP400951TKF
CSB-BP400951TKF
CSB-MP400951TKF
CSB-EP400951TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP400952TKF
CSB-EP400952TKF
CSB-BP400952TKF
CSB-MP400952TKF
CSB-EP400952TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila UPF0145 protein Tpet_0165 (Tpet_0165) CSB-YP400953TKF
CSB-EP400953TKF
CSB-BP400953TKF
CSB-MP400953TKF
CSB-EP400953TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Cysteine--tRNA ligase (cysS) CSB-YP400954TKF
CSB-EP400954TKF
CSB-BP400954TKF
CSB-MP400954TKF
CSB-EP400954TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila UPF0133 protein Tpet_0244 (Tpet_0244) CSB-YP400955TKF
CSB-EP400955TKF
CSB-BP400955TKF
CSB-MP400955TKF
CSB-EP400955TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP400956TKF
CSB-EP400956TKF
CSB-BP400956TKF
CSB-MP400956TKF
CSB-EP400956TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Probable transcriptional regulatory protein Tpet_0454 (Tpet_0454) CSB-YP400957TKF
CSB-EP400957TKF
CSB-BP400957TKF
CSB-MP400957TKF
CSB-EP400957TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila 50S ribosomal protein L33 (rpmG) CSB-YP400958TKF
CSB-EP400958TKF
CSB-BP400958TKF
CSB-MP400958TKF
CSB-EP400958TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Probable endonuclease 4 (nfo) CSB-YP400959TKF
CSB-EP400959TKF
CSB-BP400959TKF
CSB-MP400959TKF
CSB-EP400959TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Dihydroorotate dehydrogenase B (NAD (+)), catalytic subunit CSB-YP400960TKF
CSB-EP400960TKF
CSB-BP400960TKF
CSB-MP400960TKF
CSB-EP400960TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila UvrABC system protein C (uvrC), partial CSB-YP400961TKF
CSB-EP400961TKF
CSB-BP400961TKF
CSB-MP400961TKF
CSB-EP400961TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP400962TKF
CSB-EP400962TKF
CSB-BP400962TKF
CSB-MP400962TKF
CSB-EP400962TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Non-canonical purine NTP pyrophosphatase (Tpet_0766) CSB-YP400963TKF
CSB-EP400963TKF
CSB-BP400963TKF
CSB-MP400963TKF
CSB-EP400963TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP400964TKF
CSB-EP400964TKF
CSB-BP400964TKF
CSB-MP400964TKF
CSB-EP400964TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila GTP cyclohydrolase folE2 (folE2) CSB-YP400965TKF
CSB-EP400965TKF
CSB-BP400965TKF
CSB-MP400965TKF
CSB-EP400965TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Endonuclease V (nfi) CSB-YP400966TKF
CSB-EP400966TKF
CSB-BP400966TKF
CSB-MP400966TKF
CSB-EP400966TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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