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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Clostridium botulinum ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP401627CWV
CSB-BP401627CWV
CSB-MP401627CWV
CSB-EP401627CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 10 kDa chaperonin (groS) CSB-YP401628CWV
CSB-EP401628CWV
CSB-BP401628CWV
CSB-MP401628CWV
CSB-EP401628CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP401629CWV
CSB-EP401629CWV
CSB-BP401629CWV
CSB-MP401629CWV
CSB-EP401629CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L18 (rplR) CSB-YP401630CWV
CSB-EP401630CWV
CSB-BP401630CWV
CSB-MP401630CWV
CSB-EP401630CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L29 (rpmC) CSB-YP401631CWV
CSB-EP401631CWV
CSB-BP401631CWV
CSB-MP401631CWV
CSB-EP401631CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L4 (rplD) CSB-YP401632CWV
CSB-EP401632CWV
CSB-BP401632CWV
CSB-MP401632CWV
CSB-EP401632CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP401633CWV
CSB-EP401633CWV
CSB-BP401633CWV
CSB-MP401633CWV
CSB-EP401633CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP401634CWV
CSB-EP401634CWV
CSB-BP401634CWV
CSB-MP401634CWV
CSB-EP401634CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Peptidyl-tRNA hydrolase (pth) CSB-YP401635CWV
CSB-EP401635CWV
CSB-BP401635CWV
CSB-MP401635CWV
CSB-EP401635CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP401636CWV
CSB-EP401636CWV
CSB-BP401636CWV
CSB-MP401636CWV
CSB-EP401636CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP401637CWV
CSB-EP401637CWV
CSB-BP401637CWV
CSB-MP401637CWV
CSB-EP401637CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila UPF0178 protein LPC_0108 (LPC_0108) CSB-YP401638LLJ
CSB-EP401638LLJ
CSB-BP401638LLJ
CSB-MP401638LLJ
CSB-EP401638LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Dihydrodipicolinate reductase (dapB) CSB-YP401639LLJ
CSB-EP401639LLJ
CSB-BP401639LLJ
CSB-MP401639LLJ
CSB-EP401639LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP401640LLJ
CSB-EP401640LLJ
CSB-BP401640LLJ
CSB-MP401640LLJ
CSB-EP401640LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP401641LLJ
CSB-EP401641LLJ
CSB-BP401641LLJ
CSB-MP401641LLJ
CSB-EP401641LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Transcription elongation factor GreA (greA) CSB-YP401642LLJ
CSB-EP401642LLJ
CSB-BP401642LLJ
CSB-MP401642LLJ
CSB-EP401642LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Probable potassium transport system protein kup 1 (kup1), partial CSB-YP401643LLJ
CSB-EP401643LLJ
CSB-BP401643LLJ
CSB-MP401643LLJ
CSB-EP401643LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP401644LLJ
CSB-EP401644LLJ
CSB-BP401644LLJ
CSB-MP401644LLJ
CSB-EP401644LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Ribonuclease H (rnhA) CSB-YP401645LLJ
CSB-EP401645LLJ
CSB-BP401645LLJ
CSB-MP401645LLJ
CSB-EP401645LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Protein translocase subunit SecA (secA), partial CSB-YP401646LLJ
CSB-EP401646LLJ
CSB-BP401646LLJ
CSB-MP401646LLJ
CSB-EP401646LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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