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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechococcus sp. Cytochrome c biogenesis protein CcsB (ccsB), partial CSB-YP401227SVB1
CSB-EP401227SVB1
CSB-BP401227SVB1
CSB-MP401227SVB1
CSB-EP401227SVB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phycoerythrobilin:ferredoxin oxidoreductase (pebB) CSB-YP401228SVB
CSB-EP401228SVB
CSB-BP401228SVB
CSB-MP401228SVB
CSB-EP401228SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L22 (rplV) CSB-YP401229SVB
CSB-EP401229SVB
CSB-BP401229SVB
CSB-MP401229SVB
CSB-EP401229SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S8 (rpsH) CSB-YP401230SVB
CSB-EP401230SVB
CSB-BP401230SVB
CSB-MP401230SVB
CSB-EP401230SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L15 (rplO) CSB-YP401231SVB
CSB-EP401231SVB
CSB-BP401231SVB
CSB-MP401231SVB
CSB-EP401231SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L17 (rplQ) CSB-YP401232SVB
CSB-EP401232SVB
CSB-BP401232SVB
CSB-MP401232SVB
CSB-EP401232SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Aspartate carbamoyltransferase (pyrB) CSB-YP401233SVB
CSB-EP401233SVB
CSB-BP401233SVB
CSB-MP401233SVB
CSB-EP401233SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP401234SVB
CSB-EP401234SVB
CSB-BP401234SVB
CSB-MP401234SVB
CSB-EP401234SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem II reaction center protein H (psbH), partial CSB-YP401235SVB1
CSB-EP401235SVB1
CSB-BP401235SVB1
CSB-MP401235SVB1
CSB-EP401235SVB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Biosynthetic arginine decarboxylase (speA), partial CSB-YP401236SVB
CSB-EP401236SVB
CSB-BP401236SVB
CSB-MP401236SVB
CSB-EP401236SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. DNA ligase (ligA), partial CSB-YP401237SVB
CSB-EP401237SVB
CSB-BP401237SVB
CSB-MP401237SVB
CSB-EP401237SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lachancea kluyveri Urea amidolyase (DUR1,2), partial CSB-YP401238LMA
CSB-EP401238LMA
CSB-BP401238LMA
CSB-MP401238LMA
CSB-EP401238LMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant WU polyomavirus Large T antigen, partial CSB-YP401239WBG
CSB-EP401239WBG
CSB-BP401239WBG
CSB-MP401239WBG
CSB-EP401239WBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Chromosomal replication initiator protein DnaA (dnaA) CSB-YP401240CWV
CSB-EP401240CWV
CSB-BP401240CWV
CSB-MP401240CWV
CSB-EP401240CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0133 protein CBO0032/CLC_0052 (CBO0032, CLC_0052) CSB-YP401241CWV
CSB-EP401241CWV
CSB-BP401241CWV
CSB-MP401241CWV
CSB-EP401241CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum S-adenosylmethionine synthase (metK) CSB-YP401242CWV
CSB-EP401242CWV
CSB-BP401242CWV
CSB-MP401242CWV
CSB-EP401242CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0059 membrane protein CBO0394 (CBO0394), partial CSB-YP401243CWV1
CSB-EP401243CWV1
CSB-BP401243CWV1
CSB-MP401243CWV1
CSB-EP401243CWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0316 protein CBO0591/CLC_0646 (CBO0591, CLC_0646), partial CSB-YP401244CWV1
CSB-EP401244CWV1
CSB-BP401244CWV1
CSB-MP401244CWV1
CSB-EP401244CWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP401245CWV
CSB-EP401245CWV
CSB-BP401245CWV
CSB-MP401245CWV
CSB-EP401245CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Bifunctional protein FolD (folD) CSB-YP401246CWV
CSB-EP401246CWV
CSB-BP401246CWV
CSB-MP401246CWV
CSB-EP401246CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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