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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Geobacter uraniireducens Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP401147GEI
CSB-EP401147GEI
CSB-BP401147GEI
CSB-MP401147GEI
CSB-EP401147GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Protein-L-isoaspartate O-methyltransferase 2 (pcm2) CSB-YP401148GEI
CSB-EP401148GEI
CSB-BP401148GEI
CSB-MP401148GEI
CSB-EP401148GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Phosphoglucosamine mutase (glmM) CSB-YP401149GEI
CSB-EP401149GEI
CSB-BP401149GEI
CSB-MP401149GEI
CSB-EP401149GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP401150GEI
CSB-EP401150GEI
CSB-BP401150GEI
CSB-MP401150GEI
CSB-EP401150GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Leucine--tRNA ligase (leuS), partial CSB-YP401151GEI
CSB-EP401151GEI
CSB-BP401151GEI
CSB-MP401151GEI
CSB-EP401151GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Tryptophan synthase alpha chain (trpA) CSB-YP401152GEI
CSB-EP401152GEI
CSB-BP401152GEI
CSB-MP401152GEI
CSB-EP401152GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP401153GEI
CSB-EP401153GEI
CSB-BP401153GEI
CSB-MP401153GEI
CSB-EP401153GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Maf-like protein Gura_3686 (Gura_3686) CSB-YP401154GEI
CSB-EP401154GEI
CSB-BP401154GEI
CSB-MP401154GEI
CSB-EP401154GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 30S ribosomal protein S16 (rpsP) CSB-YP401155GEI
CSB-EP401155GEI
CSB-BP401155GEI
CSB-MP401155GEI
CSB-EP401155GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP401156GEI
CSB-EP401156GEI
CSB-BP401156GEI
CSB-MP401156GEI
CSB-EP401156GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP401157GEI
CSB-EP401157GEI
CSB-BP401157GEI
CSB-MP401157GEI
CSB-EP401157GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Flagellar P-ring protein (flgI) CSB-YP401158GEI
CSB-EP401158GEI
CSB-BP401158GEI
CSB-MP401158GEI
CSB-EP401158GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP401159GEI
CSB-EP401159GEI
CSB-BP401159GEI
CSB-MP401159GEI
CSB-EP401159GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Ribosomal protein L11 methyltransferase (prmA) CSB-YP401160GEI
CSB-EP401160GEI
CSB-BP401160GEI
CSB-MP401160GEI
CSB-EP401160GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP401161GEI
CSB-EP401161GEI
CSB-BP401161GEI
CSB-MP401161GEI
CSB-EP401161GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP401162GEI
CSB-EP401162GEI
CSB-BP401162GEI
CSB-MP401162GEI
CSB-EP401162GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens DNA ligase (ligA), partial CSB-YP401163GEI
CSB-EP401163GEI
CSB-BP401163GEI
CSB-MP401163GEI
CSB-EP401163GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Protease HtpX homolog (htpX), partial CSB-YP401164GEI1
CSB-EP401164GEI1
CSB-BP401164GEI1
CSB-MP401164GEI1
CSB-EP401164GEI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 50S ribosomal protein L17 (rplQ) CSB-YP401165GEI
CSB-EP401165GEI
CSB-BP401165GEI
CSB-MP401165GEI
CSB-EP401165GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 50S ribosomal protein L6 (rplF) CSB-YP401166GEI
CSB-EP401166GEI
CSB-BP401166GEI
CSB-MP401166GEI
CSB-EP401166GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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