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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus 50S ribosomal protein L1 (rplA) CSB-YP401347SUJ
CSB-EP401347SUJ
CSB-BP401347SUJ
CSB-MP401347SUJ
CSB-EP401347SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S7 (rpsG) CSB-YP401348SUJ
CSB-EP401348SUJ
CSB-BP401348SUJ
CSB-MP401348SUJ
CSB-EP401348SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0447 protein SaurJH9_0610 (SaurJH9_0610) CSB-YP401349SUJ
CSB-EP401349SUJ
CSB-BP401349SUJ
CSB-MP401349SUJ
CSB-EP401349SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable transcriptional regulatory protein SaurJH9_0693 (SaurJH9_0693) CSB-YP401350SUJ
CSB-EP401350SUJ
CSB-BP401350SUJ
CSB-MP401350SUJ
CSB-EP401350SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP401351SUJ
CSB-EP401351SUJ
CSB-BP401351SUJ
CSB-MP401351SUJ
CSB-EP401351SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP401352SUJ1
CSB-EP401352SUJ1
CSB-BP401352SUJ1
CSB-MP401352SUJ1
CSB-EP401352SUJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus SsrA-binding protein (smpB) CSB-YP401353SUJ
CSB-EP401353SUJ
CSB-BP401353SUJ
CSB-MP401353SUJ
CSB-EP401353SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0349 protein SaurJH9_0939 (SaurJH9_0939) CSB-YP401354SUJ
CSB-EP401354SUJ
CSB-BP401354SUJ
CSB-MP401354SUJ
CSB-EP401354SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP-dependent helicase/deoxyribonuclease subunit B (addB), partial CSB-YP401355SUJ
CSB-EP401355SUJ
CSB-BP401355SUJ
CSB-MP401355SUJ
CSB-EP401355SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Processive diacylglycerol glucosyltransferase (ugtP) CSB-YP401356SUJ
CSB-EP401356SUJ
CSB-BP401356SUJ
CSB-MP401356SUJ
CSB-EP401356SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP401357SUJ
CSB-EP401357SUJ
CSB-BP401357SUJ
CSB-MP401357SUJ
CSB-EP401357SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Iron-regulated surface determinant protein A (isdA) CSB-YP401358SUJ
CSB-EP401358SUJ
CSB-BP401358SUJ
CSB-MP401358SUJ
CSB-EP401358SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus MutS2 protein (mutS2), partial CSB-YP401359SUJ
CSB-EP401359SUJ
CSB-BP401359SUJ
CSB-MP401359SUJ
CSB-EP401359SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP401360SUJ
CSB-EP401360SUJ
CSB-BP401360SUJ
CSB-MP401360SUJ
CSB-EP401360SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Aspartate carbamoyltransferase (pyrB) CSB-YP401361SUJ
CSB-EP401361SUJ
CSB-BP401361SUJ
CSB-MP401361SUJ
CSB-EP401361SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphate acyltransferase (plsX) CSB-YP401362SUJ
CSB-EP401362SUJ
CSB-BP401362SUJ
CSB-MP401362SUJ
CSB-EP401362SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L19 (rplS) CSB-YP401363SUJ
CSB-EP401363SUJ
CSB-BP401363SUJ
CSB-MP401363SUJ
CSB-EP401363SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus tRNA pseudouridine synthase B (truB) CSB-YP401364SUJ
CSB-EP401364SUJ
CSB-BP401364SUJ
CSB-MP401364SUJ
CSB-EP401364SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glycerol kinase (glpK) CSB-YP401365SUJ
CSB-EP401365SUJ
CSB-BP401365SUJ
CSB-MP401365SUJ
CSB-EP401365SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Nuclease sbcCD subunit C (sbcC), partial CSB-YP401366SUJ
CSB-EP401366SUJ
CSB-BP401366SUJ
CSB-MP401366SUJ
CSB-EP401366SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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