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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Legionella pneumophila Alanine--tRNA ligase (alaS), partial CSB-YP402348LLJ
CSB-EP402348LLJ
CSB-BP402348LLJ
CSB-MP402348LLJ
CSB-EP402348LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP402349LLJ
CSB-EP402349LLJ
CSB-BP402349LLJ
CSB-MP402349LLJ
CSB-EP402349LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila UPF0434 protein LPC_1374 (LPC_1374) CSB-YP402350LLJ
CSB-EP402350LLJ
CSB-BP402350LLJ
CSB-MP402350LLJ
CSB-EP402350LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Chaperone protein DnaJ (dnaJ) CSB-YP402351LLJ
CSB-EP402351LLJ
CSB-BP402351LLJ
CSB-MP402351LLJ
CSB-EP402351LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP402352LLJ1
CSB-EP402352LLJ1
CSB-BP402352LLJ1
CSB-MP402352LLJ1
CSB-EP402352LLJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Catalase-peroxidase 1 (katG1), partial CSB-YP402353LLJ
CSB-EP402353LLJ
CSB-BP402353LLJ
CSB-MP402353LLJ
CSB-EP402353LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Isoleucine--tRNA ligase (ileS), partial CSB-YP402354LLJ
CSB-EP402354LLJ
CSB-BP402354LLJ
CSB-MP402354LLJ
CSB-EP402354LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP402355LLJ
CSB-EP402355LLJ
CSB-BP402355LLJ
CSB-MP402355LLJ
CSB-EP402355LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP402356LLJ
CSB-EP402356LLJ
CSB-BP402356LLJ
CSB-MP402356LLJ
CSB-EP402356LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 10 kDa chaperonin (groS) CSB-YP402357LLJ
CSB-EP402357LLJ
CSB-BP402357LLJ
CSB-MP402357LLJ
CSB-EP402357LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Carboxylate-amine ligase LPC_2680 (LPC_2680) CSB-YP402358LLJ
CSB-EP402358LLJ
CSB-BP402358LLJ
CSB-MP402358LLJ
CSB-EP402358LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP402359LLJ
CSB-EP402359LLJ
CSB-BP402359LLJ
CSB-MP402359LLJ
CSB-EP402359LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Ribosomal protein S6 modification protein (rimK) CSB-YP402360LLJ
CSB-EP402360LLJ
CSB-BP402360LLJ
CSB-MP402360LLJ
CSB-EP402360LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP402361LLJ
CSB-EP402361LLJ
CSB-BP402361LLJ
CSB-MP402361LLJ
CSB-EP402361LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 30S ribosomal protein S5 (rpsE) CSB-YP402362LLJ
CSB-EP402362LLJ
CSB-BP402362LLJ
CSB-MP402362LLJ
CSB-EP402362LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 50S ribosomal protein L14 (rplN) CSB-YP402363LLJ
CSB-EP402363LLJ
CSB-BP402363LLJ
CSB-MP402363LLJ
CSB-EP402363LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 30S ribosomal protein S19 (rpsS) CSB-YP402364LLJ
CSB-EP402364LLJ
CSB-BP402364LLJ
CSB-MP402364LLJ
CSB-EP402364LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 50S ribosomal protein L7/L12 (rplL) CSB-YP402365LLJ
CSB-EP402365LLJ
CSB-BP402365LLJ
CSB-MP402365LLJ
CSB-EP402365LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP402366LLJ
CSB-EP402366LLJ
CSB-BP402366LLJ
CSB-MP402366LLJ
CSB-EP402366LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP402367LLJ
CSB-EP402367LLJ
CSB-BP402367LLJ
CSB-MP402367LLJ
CSB-EP402367LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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