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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus DNA ligase (ligA), partial CSB-YP401747SUJ
CSB-EP401747SUJ
CSB-BP401747SUJ
CSB-MP401747SUJ
CSB-EP401747SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Accessory gene regulator protein B (agrB), partial CSB-YP401748SUJ1
CSB-EP401748SUJ1
CSB-BP401748SUJ1
CSB-MP401748SUJ1
CSB-EP401748SUJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Membrane protein insertase YidC (yidC), partial CSB-YP401749SUJ1
CSB-EP401749SUJ1
CSB-BP401749SUJ1
CSB-MP401749SUJ1
CSB-EP401749SUJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP synthase epsilon chain (atpC) CSB-YP401750SUJ
CSB-EP401750SUJ
CSB-BP401750SUJ
CSB-MP401750SUJ
CSB-EP401750SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP synthase subunit c (atpE), partial CSB-YP401751SUJ1
CSB-EP401751SUJ1
CSB-BP401751SUJ1
CSB-MP401751SUJ1
CSB-EP401751SUJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable DNA-directed RNA polymerase subunit delta (rpoE) CSB-YP401752SUJ
CSB-EP401752SUJ
CSB-BP401752SUJ
CSB-MP401752SUJ
CSB-EP401752SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L13 (rplM) CSB-YP401753SUJ
CSB-EP401753SUJ
CSB-BP401753SUJ
CSB-MP401753SUJ
CSB-EP401753SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L30 (rpmD) CSB-YP401754SUJ
CSB-EP401754SUJ
CSB-BP401754SUJ
CSB-MP401754SUJ
CSB-EP401754SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S17 (rpsQ) CSB-YP401755SUJ
CSB-EP401755SUJ
CSB-BP401755SUJ
CSB-MP401755SUJ
CSB-EP401755SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Urease subunit gamma (ureA) CSB-YP401756SUJ
CSB-EP401756SUJ
CSB-BP401756SUJ
CSB-MP401756SUJ
CSB-EP401756SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Imidazolonepropionase (hutI) CSB-YP401757SUJ
CSB-EP401757SUJ
CSB-BP401757SUJ
CSB-MP401757SUJ
CSB-EP401757SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Heme sensor protein hssS (hssS), partial CSB-YP401758SUJ1
CSB-EP401758SUJ1
CSB-BP401758SUJ1
CSB-MP401758SUJ1
CSB-EP401758SUJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 1-pyrroline-5-carboxylate dehydrogenase (rocA), partial CSB-YP401759SUJ
CSB-EP401759SUJ
CSB-BP401759SUJ
CSB-MP401759SUJ
CSB-EP401759SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus L-lactate dehydrogenase 2 (ldh2) CSB-YP401760SUJ
CSB-EP401760SUJ
CSB-BP401760SUJ
CSB-MP401760SUJ
CSB-EP401760SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP401761SUJ
CSB-EP401761SUJ
CSB-BP401761SUJ
CSB-MP401761SUJ
CSB-EP401761SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Pyrrolidone-carboxylate peptidase (pcp) CSB-YP401762SUJ
CSB-EP401762SUJ
CSB-BP401762SUJ
CSB-MP401762SUJ
CSB-EP401762SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP401763MWC
CSB-EP401763MWC
CSB-BP401763MWC
CSB-MP401763MWC
CSB-EP401763MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP401764MWC
CSB-EP401764MWC
CSB-BP401764MWC
CSB-MP401764MWC
CSB-EP401764MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae DNA ligase (ligA), partial CSB-YP401765MWC
CSB-EP401765MWC
CSB-BP401765MWC
CSB-MP401765MWC
CSB-EP401765MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae Lon protease (lon), partial CSB-YP401766MWC
CSB-EP401766MWC
CSB-BP401766MWC
CSB-MP401766MWC
CSB-EP401766MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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