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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechococcus sp. CinA-like protein (SynWH7803_0305) CSB-YP401547SVC
CSB-EP401547SVC
CSB-BP401547SVC
CSB-MP401547SVC
CSB-EP401547SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. tRNA (guanine-N (7)-)-methyltransferase CSB-YP401548SVC
CSB-EP401548SVC
CSB-BP401548SVC
CSB-MP401548SVC
CSB-EP401548SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S10 (rpsJ) CSB-YP401549SVC
CSB-EP401549SVC
CSB-BP401549SVC
CSB-MP401549SVC
CSB-EP401549SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S5 (rpsE) CSB-YP401550SVC
CSB-EP401550SVC
CSB-BP401550SVC
CSB-MP401550SVC
CSB-EP401550SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L23 (rplW) CSB-YP401551SVC
CSB-EP401551SVC
CSB-BP401551SVC
CSB-MP401551SVC
CSB-EP401551SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP401552SVC
CSB-EP401552SVC
CSB-BP401552SVC
CSB-MP401552SVC
CSB-EP401552SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Acetylglutamate kinase (argB) CSB-YP401553SVC
CSB-EP401553SVC
CSB-BP401553SVC
CSB-MP401553SVC
CSB-EP401553SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. ATP phosphoribosyltransferase (hisG) CSB-YP401554SVC
CSB-EP401554SVC
CSB-BP401554SVC
CSB-MP401554SVC
CSB-EP401554SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Homoserine kinase (thrB) CSB-YP401555SVC
CSB-EP401555SVC
CSB-BP401555SVC
CSB-MP401555SVC
CSB-EP401555SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP401556SVC
CSB-EP401556SVC
CSB-BP401556SVC
CSB-MP401556SVC
CSB-EP401556SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem I reaction center subunit PsaK (psaK), partial CSB-YP401557SVC1
CSB-EP401557SVC1
CSB-BP401557SVC1
CSB-MP401557SVC1
CSB-EP401557SVC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem II reaction center Psb28 protein (psb28) CSB-YP401558SVC
CSB-EP401558SVC
CSB-BP401558SVC
CSB-MP401558SVC
CSB-EP401558SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 3',5'-cyclic adenosine monophosphate phosphodiesterase CpdA (cpdA) CSB-YP401559SVC
CSB-EP401559SVC
CSB-BP401559SVC
CSB-MP401559SVC
CSB-EP401559SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Bifunctional protein FolD (folD) CSB-YP401560SVC
CSB-EP401560SVC
CSB-BP401560SVC
CSB-MP401560SVC
CSB-EP401560SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP401561SVC
CSB-EP401561SVC
CSB-BP401561SVC
CSB-MP401561SVC
CSB-EP401561SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Putative pterin-4-alpha-carbinolamine dehydratase (SynWH7803_1805) CSB-YP401562SVC
CSB-EP401562SVC
CSB-BP401562SVC
CSB-MP401562SVC
CSB-EP401562SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Apocytochrome f (petA), partial CSB-YP401563SVC1
CSB-EP401563SVC1
CSB-BP401563SVC1
CSB-MP401563SVC1
CSB-EP401563SVC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L21 (rplU) CSB-YP401564SVC
CSB-EP401564SVC
CSB-BP401564SVC
CSB-MP401564SVC
CSB-EP401564SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 10 kDa chaperonin (groS) CSB-YP401565SVC
CSB-EP401565SVC
CSB-BP401565SVC
CSB-MP401565SVC
CSB-EP401565SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP401566SVC
CSB-EP401566SVC
CSB-BP401566SVC
CSB-MP401566SVC
CSB-EP401566SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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