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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechococcus sp. Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP401587SVB
CSB-EP401587SVB
CSB-BP401587SVB
CSB-MP401587SVB
CSB-EP401587SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP401588SVB
CSB-EP401588SVB
CSB-BP401588SVB
CSB-MP401588SVB
CSB-EP401588SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Light-independent protochlorophyllide reductase subunit B (chlB), partial CSB-YP401589SVB
CSB-EP401589SVB
CSB-BP401589SVB
CSB-MP401589SVB
CSB-EP401589SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. tRNA (guanine-N (1)-)-methyltransferase CSB-YP401590SVB
CSB-EP401590SVB
CSB-BP401590SVB
CSB-MP401590SVB
CSB-EP401590SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. ATP synthase gamma chain (atpG) CSB-YP401591SVB
CSB-EP401591SVB
CSB-BP401591SVB
CSB-MP401591SVB
CSB-EP401591SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP401592SVB
CSB-EP401592SVB
CSB-BP401592SVB
CSB-MP401592SVB
CSB-EP401592SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. NAD (P)H-quinone oxidoreductase subunit N CSB-YP401593SVB
CSB-EP401593SVB
CSB-BP401593SVB
CSB-MP401593SVB
CSB-EP401593SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S3 (rpsC) CSB-YP401594SVB
CSB-EP401594SVB
CSB-BP401594SVB
CSB-MP401594SVB
CSB-EP401594SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L36 (rpmJ) CSB-YP401595SVB
CSB-EP401595SVB
CSB-BP401595SVB
CSB-MP401595SVB
CSB-EP401595SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L13 (rplM) CSB-YP401596SVB
CSB-EP401596SVB
CSB-BP401596SVB
CSB-MP401596SVB
CSB-EP401596SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP401597SVB
CSB-EP401597SVB
CSB-BP401597SVB
CSB-MP401597SVB
CSB-EP401597SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Arginine--tRNA ligase (argS), partial CSB-YP401598SVB
CSB-EP401598SVB
CSB-BP401598SVB
CSB-MP401598SVB
CSB-EP401598SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem II reaction center protein K (psbK) CSB-YP401599SVB
CSB-EP401599SVB
CSB-BP401599SVB
CSB-MP401599SVB
CSB-EP401599SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Protein psbN (psbN), partial CSB-YP401600SVB
CSB-EP401600SVB
CSB-BP401600SVB
CSB-MP401600SVB
CSB-EP401600SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Alanine--tRNA ligase (alaS), partial CSB-YP401601SVB
CSB-EP401601SVB
CSB-BP401601SVB
CSB-MP401601SVB
CSB-EP401601SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Urease subunit gamma (ureA) CSB-YP401602SVB
CSB-EP401602SVB
CSB-BP401602SVB
CSB-MP401602SVB
CSB-EP401602SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP401603SVB
CSB-EP401603SVB
CSB-BP401603SVB
CSB-MP401603SVB
CSB-EP401603SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteriocin uberolysin (ublA) CSB-YP401604SUU
CSB-EP401604SUU
CSB-BP401604SUU
CSB-MP401604SUU
CSB-EP401604SUU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP401605CWV
CSB-EP401605CWV
CSB-BP401605CWV
CSB-MP401605CWV
CSB-EP401605CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Uracil phosphoribosyltransferase (upp) CSB-YP401606CWV
CSB-EP401606CWV
CSB-BP401606CWV
CSB-MP401606CWV
CSB-EP401606CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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