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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermotoga petrophila Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP401687TKF
CSB-EP401687TKF
CSB-BP401687TKF
CSB-MP401687TKF
CSB-EP401687TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP401688TKF
CSB-EP401688TKF
CSB-BP401688TKF
CSB-MP401688TKF
CSB-EP401688TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP401689TKF
CSB-EP401689TKF
CSB-BP401689TKF
CSB-MP401689TKF
CSB-EP401689TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Tryptophan synthase alpha chain (trpA) CSB-YP401690TKF
CSB-EP401690TKF
CSB-BP401690TKF
CSB-MP401690TKF
CSB-EP401690TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila UPF0103 protein Tpet_0837 (Tpet_0837) CSB-YP401691TKF
CSB-EP401691TKF
CSB-BP401691TKF
CSB-MP401691TKF
CSB-EP401691TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Glutamate--tRNA ligase 1 (gltX1) CSB-YP401692TKF
CSB-EP401692TKF
CSB-BP401692TKF
CSB-MP401692TKF
CSB-EP401692TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Ribonuclease Y (rny), partial CSB-YP401693TKF
CSB-EP401693TKF
CSB-BP401693TKF
CSB-MP401693TKF
CSB-EP401693TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila UvrABC system protein B (uvrB), partial CSB-YP401694TKF
CSB-EP401694TKF
CSB-BP401694TKF
CSB-MP401694TKF
CSB-EP401694TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP401695TKF
CSB-EP401695TKF
CSB-BP401695TKF
CSB-MP401695TKF
CSB-EP401695TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Bifunctional protein GlmU (glmU) CSB-YP401696TKF
CSB-EP401696TKF
CSB-BP401696TKF
CSB-MP401696TKF
CSB-EP401696TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Ribosome maturation factor RimM (rimM) CSB-YP401697TKF
CSB-EP401697TKF
CSB-BP401697TKF
CSB-MP401697TKF
CSB-EP401697TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Flagellar P-ring protein (flgI) CSB-YP401698TKF
CSB-EP401698TKF
CSB-BP401698TKF
CSB-MP401698TKF
CSB-EP401698TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila 30S ribosomal protein S7 (rpsG) CSB-YP401699TKF
CSB-EP401699TKF
CSB-BP401699TKF
CSB-MP401699TKF
CSB-EP401699TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila 50S ribosomal protein L29 (rpmC) CSB-YP401700TKF
CSB-EP401700TKF
CSB-BP401700TKF
CSB-MP401700TKF
CSB-EP401700TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila 50S ribosomal protein L18 (rplR) CSB-YP401701TKF
CSB-EP401701TKF
CSB-BP401701TKF
CSB-MP401701TKF
CSB-EP401701TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP401702TKF
CSB-EP401702TKF
CSB-BP401702TKF
CSB-MP401702TKF
CSB-EP401702TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila 30S ribosomal protein S9 (rpsI) CSB-YP401703TKF
CSB-EP401703TKF
CSB-BP401703TKF
CSB-MP401703TKF
CSB-EP401703TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP401704TKF
CSB-EP401704TKF
CSB-BP401704TKF
CSB-MP401704TKF
CSB-EP401704TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP401705TKF
CSB-EP401705TKF
CSB-BP401705TKF
CSB-MP401705TKF
CSB-EP401705TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Probable septum site-determining protein MinC (minC) CSB-YP401706TKF
CSB-EP401706TKF
CSB-BP401706TKF
CSB-MP401706TKF
CSB-EP401706TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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