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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus Ribonuclease HII (rnhB) CSB-YP401727SUJ
CSB-EP401727SUJ
CSB-BP401727SUJ
CSB-MP401727SUJ
CSB-EP401727SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Proline--tRNA ligase (proS), partial CSB-YP401728SUJ
CSB-EP401728SUJ
CSB-BP401728SUJ
CSB-MP401728SUJ
CSB-EP401728SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP401729SUJ
CSB-EP401729SUJ
CSB-BP401729SUJ
CSB-MP401729SUJ
CSB-EP401729SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP401730SUJ1
CSB-EP401730SUJ1
CSB-BP401730SUJ1
CSB-MP401730SUJ1
CSB-EP401730SUJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Tryptophan synthase alpha chain (trpA) CSB-YP401731SUJ
CSB-EP401731SUJ
CSB-BP401731SUJ
CSB-MP401731SUJ
CSB-EP401731SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0346 protein SaurJH9_1481 (SaurJH9_1481) CSB-YP401732SUJ
CSB-EP401732SUJ
CSB-BP401732SUJ
CSB-MP401732SUJ
CSB-EP401732SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP401733SUJ
CSB-EP401733SUJ
CSB-BP401733SUJ
CSB-MP401733SUJ
CSB-EP401733SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribonuclease Z (rnz) CSB-YP401734SUJ
CSB-EP401734SUJ
CSB-BP401734SUJ
CSB-MP401734SUJ
CSB-EP401734SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB) CSB-YP401735SUJ
CSB-EP401735SUJ
CSB-BP401735SUJ
CSB-MP401735SUJ
CSB-EP401735SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative phosphotransferase SaurJH9_1621 (SaurJH9_1621) CSB-YP401736SUJ
CSB-EP401736SUJ
CSB-BP401736SUJ
CSB-MP401736SUJ
CSB-EP401736SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Chaperone protein DnaJ (dnaJ) CSB-YP401737SUJ
CSB-EP401737SUJ
CSB-BP401737SUJ
CSB-MP401737SUJ
CSB-EP401737SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Elongation factor 4 (lepA), partial CSB-YP401738SUJ
CSB-EP401738SUJ
CSB-BP401738SUJ
CSB-MP401738SUJ
CSB-EP401738SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Transcription elongation factor GreA (greA) CSB-YP401739SUJ
CSB-EP401739SUJ
CSB-BP401739SUJ
CSB-MP401739SUJ
CSB-EP401739SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP401740SUJ
CSB-EP401740SUJ
CSB-BP401740SUJ
CSB-MP401740SUJ
CSB-EP401740SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Valine--tRNA ligase (valS), partial CSB-YP401741SUJ
CSB-EP401741SUJ
CSB-BP401741SUJ
CSB-MP401741SUJ
CSB-EP401741SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Trigger factor (tig) CSB-YP401742SUJ
CSB-EP401742SUJ
CSB-BP401742SUJ
CSB-MP401742SUJ
CSB-EP401742SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Tyrosine--tRNA ligase (tyrS) CSB-YP401743SUJ
CSB-EP401743SUJ
CSB-BP401743SUJ
CSB-MP401743SUJ
CSB-EP401743SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Leucine--tRNA ligase (leuS), partial CSB-YP401744SUJ
CSB-EP401744SUJ
CSB-BP401744SUJ
CSB-MP401744SUJ
CSB-EP401744SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine protease splC (splC) CSB-YP401745SUJ
CSB-EP401745SUJ
CSB-BP401745SUJ
CSB-MP401745SUJ
CSB-EP401745SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0374 protein SaurJH9_1921 (SaurJH9_1921) CSB-YP401746SUJ
CSB-EP401746SUJ
CSB-BP401746SUJ
CSB-MP401746SUJ
CSB-EP401746SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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