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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechococcus sp. 7-cyano-7-deazaguanine synthase (queC) CSB-YP401968SVB
CSB-EP401968SVB
CSB-BP401968SVB
CSB-MP401968SVB
CSB-EP401968SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S6 (rpsF) CSB-YP401969SVB
CSB-EP401969SVB
CSB-BP401969SVB
CSB-MP401969SVB
CSB-EP401969SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Pentafunctional AROM polypeptide 1 (ARO1-1), partial CSB-YP401970LLV
CSB-EP401970LLV
CSB-BP401970LLV
CSB-MP401970LLV
CSB-EP401970LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant WU polyomavirus Small t antigen CSB-YP401971WBG
CSB-EP401971WBG
CSB-BP401971WBG
CSB-MP401971WBG
CSB-EP401971WBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribonuclease M5 (rnmV) CSB-YP401972CWV
CSB-EP401972CWV
CSB-BP401972CWV
CSB-MP401972CWV
CSB-EP401972CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum ATP synthase subunit c (atpE), partial CSB-YP401973CWV1
CSB-EP401973CWV1
CSB-BP401973CWV1
CSB-MP401973CWV1
CSB-EP401973CWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Protein translocase subunit SecA (secA), partial CSB-YP401974CWV
CSB-EP401974CWV
CSB-BP401974CWV
CSB-MP401974CWV
CSB-EP401974CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum ATP-dependent helicase/nuclease subunit A (addA), partial CSB-YP401975CWV
CSB-EP401975CWV
CSB-BP401975CWV
CSB-MP401975CWV
CSB-EP401975CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum D-alanine--D-alanine ligase (ddl) CSB-YP401976CWV
CSB-EP401976CWV
CSB-BP401976CWV
CSB-MP401976CWV
CSB-EP401976CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum tRNA-specific 2-thiouridylase mnmA 1 (mnmA1) CSB-YP401977CWV
CSB-EP401977CWV
CSB-BP401977CWV
CSB-MP401977CWV
CSB-EP401977CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0059 membrane protein CBO1290/CLC_1328 (CBO1290, CLC_1328), partial CSB-YP401978CWV1
CSB-EP401978CWV1
CSB-BP401978CWV1
CSB-MP401978CWV1
CSB-EP401978CWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum L-lactate dehydrogenase (ldh) CSB-YP401979CWV
CSB-EP401979CWV
CSB-BP401979CWV
CSB-MP401979CWV
CSB-EP401979CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP401980CWV
CSB-EP401980CWV
CSB-BP401980CWV
CSB-MP401980CWV
CSB-EP401980CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Homoserine kinase (thrB) CSB-YP401981CWV
CSB-EP401981CWV
CSB-BP401981CWV
CSB-MP401981CWV
CSB-EP401981CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0597 protein CBO1815/CLC_1757 (CBO1815, CLC_1757) CSB-YP401982CWV
CSB-EP401982CWV
CSB-BP401982CWV
CSB-MP401982CWV
CSB-EP401982CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum N utilization substance protein B homolog (nusB) CSB-YP401983CWV
CSB-EP401983CWV
CSB-BP401983CWV
CSB-MP401983CWV
CSB-EP401983CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Hydroxyethylthiazole kinase 2 (thiM2) CSB-YP401984CWV
CSB-EP401984CWV
CSB-BP401984CWV
CSB-MP401984CWV
CSB-EP401984CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S15 (rpsO) CSB-YP401985CWV
CSB-EP401985CWV
CSB-BP401985CWV
CSB-MP401985CWV
CSB-EP401985CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribosome maturation factor RimM (rimM) CSB-YP401986CWV
CSB-EP401986CWV
CSB-BP401986CWV
CSB-MP401986CWV
CSB-EP401986CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP401987CWV
CSB-EP401987CWV
CSB-BP401987CWV
CSB-MP401987CWV
CSB-EP401987CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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