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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Legionella pneumophila Phosphoglucosamine mutase (glmM) CSB-YP402368LLJ
CSB-EP402368LLJ
CSB-BP402368LLJ
CSB-MP402368LLJ
CSB-EP402368LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP402369LLJ
CSB-EP402369LLJ
CSB-BP402369LLJ
CSB-MP402369LLJ
CSB-EP402369LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila ATP synthase epsilon chain (atpC) CSB-YP402370LLJ
CSB-EP402370LLJ
CSB-BP402370LLJ
CSB-MP402370LLJ
CSB-EP402370LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila ATP synthase subunit alpha 2 (atpA2), partial CSB-YP402371LLJ
CSB-EP402371LLJ
CSB-BP402371LLJ
CSB-MP402371LLJ
CSB-EP402371LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Ribonuclease P protein component (rnpA) CSB-YP402372LLJ
CSB-EP402372LLJ
CSB-BP402372LLJ
CSB-MP402372LLJ
CSB-EP402372LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP402373TKF
CSB-EP402373TKF
CSB-BP402373TKF
CSB-MP402373TKF
CSB-EP402373TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila 50S ribosomal protein L9 (rplI) CSB-YP402374TKF
CSB-EP402374TKF
CSB-BP402374TKF
CSB-MP402374TKF
CSB-EP402374TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Phosphomethylpyrimidine synthase (thiC) CSB-YP402375TKF
CSB-EP402375TKF
CSB-BP402375TKF
CSB-MP402375TKF
CSB-EP402375TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila D-tyrosyl-tRNA (Tyr) deacylase CSB-YP402376TKF
CSB-EP402376TKF
CSB-BP402376TKF
CSB-MP402376TKF
CSB-EP402376TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila 30S ribosomal protein S18 (rpsR) CSB-YP402377TKF
CSB-EP402377TKF
CSB-BP402377TKF
CSB-MP402377TKF
CSB-EP402377TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila 2-isopropylmalate synthase (leuA), partial CSB-YP402378TKF
CSB-EP402378TKF
CSB-BP402378TKF
CSB-MP402378TKF
CSB-EP402378TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP402379TKF
CSB-EP402379TKF
CSB-BP402379TKF
CSB-MP402379TKF
CSB-EP402379TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Thymidylate synthase thyX (thyX) CSB-YP402380TKF
CSB-EP402380TKF
CSB-BP402380TKF
CSB-MP402380TKF
CSB-EP402380TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Shikimate dehydrogenase (aroE) CSB-YP402381TKF
CSB-EP402381TKF
CSB-BP402381TKF
CSB-MP402381TKF
CSB-EP402381TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Probable transaldolase (tal) CSB-YP402382TKF
CSB-EP402382TKF
CSB-BP402382TKF
CSB-MP402382TKF
CSB-EP402382TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP402383TKF
CSB-EP402383TKF
CSB-BP402383TKF
CSB-MP402383TKF
CSB-EP402383TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP402384TKF
CSB-EP402384TKF
CSB-BP402384TKF
CSB-MP402384TKF
CSB-EP402384TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP402385TKF
CSB-EP402385TKF
CSB-BP402385TKF
CSB-MP402385TKF
CSB-EP402385TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Leucine--tRNA ligase (leuS), partial CSB-YP402386TKF
CSB-EP402386TKF
CSB-BP402386TKF
CSB-MP402386TKF
CSB-EP402386TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila 50S ribosomal protein L32 (rpmF) CSB-YP402387TKF
CSB-EP402387TKF
CSB-BP402387TKF
CSB-MP402387TKF
CSB-EP402387TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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