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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermotoga petrophila Orotate phosphoribosyltransferase (pyrE) CSB-YP402048TKF
CSB-EP402048TKF
CSB-BP402048TKF
CSB-MP402048TKF
CSB-EP402048TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila SsrA-binding protein (smpB) CSB-YP402049TKF
CSB-EP402049TKF
CSB-BP402049TKF
CSB-MP402049TKF
CSB-EP402049TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB) CSB-YP402050TKF
CSB-EP402050TKF
CSB-BP402050TKF
CSB-MP402050TKF
CSB-EP402050TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Redox-sensing transcriptional repressor rex (rex) CSB-YP402051TKF
CSB-EP402051TKF
CSB-BP402051TKF
CSB-MP402051TKF
CSB-EP402051TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP402052TKF
CSB-EP402052TKF
CSB-BP402052TKF
CSB-MP402052TKF
CSB-EP402052TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Flagellar assembly factor FliW (fliW) CSB-YP402053TKF
CSB-EP402053TKF
CSB-BP402053TKF
CSB-MP402053TKF
CSB-EP402053TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila L-lactate dehydrogenase (ldh) CSB-YP402054TKF
CSB-EP402054TKF
CSB-BP402054TKF
CSB-MP402054TKF
CSB-EP402054TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila 50S ribosomal protein L31 (rpmE) CSB-YP402055TKF
CSB-EP402055TKF
CSB-BP402055TKF
CSB-MP402055TKF
CSB-EP402055TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Transcriptional repressor NrdR (nrdR) CSB-YP402056TKF
CSB-EP402056TKF
CSB-BP402056TKF
CSB-MP402056TKF
CSB-EP402056TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila DNA mismatch repair protein MutS (mutS), partial CSB-YP402057TKF
CSB-EP402057TKF
CSB-BP402057TKF
CSB-MP402057TKF
CSB-EP402057TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Ribosome maturation factor RimP (rimP) CSB-YP402058TKF
CSB-EP402058TKF
CSB-BP402058TKF
CSB-MP402058TKF
CSB-EP402058TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila 5'-nucleotidase surE (surE) CSB-YP402059TKF
CSB-EP402059TKF
CSB-BP402059TKF
CSB-MP402059TKF
CSB-EP402059TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Peptide chain release factor 2 (prfB) CSB-YP402060TKF
CSB-EP402060TKF
CSB-BP402060TKF
CSB-MP402060TKF
CSB-EP402060TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Dihydrodipicolinate reductase (dapB) CSB-YP402061TKF
CSB-EP402061TKF
CSB-BP402061TKF
CSB-MP402061TKF
CSB-EP402061TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila 50S ribosomal protein L2 (rplB) CSB-YP402062TKF
CSB-EP402062TKF
CSB-BP402062TKF
CSB-MP402062TKF
CSB-EP402062TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila 30S ribosomal protein S11 (rpsK) CSB-YP402063TKF
CSB-EP402063TKF
CSB-BP402063TKF
CSB-MP402063TKF
CSB-EP402063TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila 50S ribosomal protein L27 (rpmA) CSB-YP402064TKF
CSB-EP402064TKF
CSB-BP402064TKF
CSB-MP402064TKF
CSB-EP402064TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP402065TKF
CSB-EP402065TKF
CSB-BP402065TKF
CSB-MP402065TKF
CSB-EP402065TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila UPF0173 metal-dependent hydrolase Tpet_1587 (Tpet_1587) CSB-YP402066TKF
CSB-EP402066TKF
CSB-BP402066TKF
CSB-MP402066TKF
CSB-EP402066TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Histidine--tRNA ligase (hisS) CSB-YP402067TKF
CSB-EP402067TKF
CSB-BP402067TKF
CSB-MP402067TKF
CSB-EP402067TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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