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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechococcus sp. Elongation factor 4 (lepA), partial CSB-YP401927SVC
CSB-EP401927SVC
CSB-BP401927SVC
CSB-MP401927SVC
CSB-EP401927SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Cytochrome b6-f complex iron-sulfur subunit (petC), partial CSB-YP401928SVC1
CSB-EP401928SVC1
CSB-BP401928SVC1
CSB-MP401928SVC1
CSB-EP401928SVC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S14 (rpsN) CSB-YP401929SVC
CSB-EP401929SVC
CSB-BP401929SVC
CSB-MP401929SVC
CSB-EP401929SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP401930SVC
CSB-EP401930SVC
CSB-BP401930SVC
CSB-MP401930SVC
CSB-EP401930SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 60 kDa chaperonin 2 (groL2), partial CSB-YP401931SVC
CSB-EP401931SVC
CSB-BP401931SVC
CSB-MP401931SVC
CSB-EP401931SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP401932SVC
CSB-EP401932SVC
CSB-BP401932SVC
CSB-MP401932SVC
CSB-EP401932SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S21 (rpsU) CSB-YP401933SVC
CSB-EP401933SVC
CSB-BP401933SVC
CSB-MP401933SVC
CSB-EP401933SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem I assembly protein ycf3 (ycf3) CSB-YP401934SVC
CSB-EP401934SVC
CSB-BP401934SVC
CSB-MP401934SVC
CSB-EP401934SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. tRNA modification GTPase MnmE (mnmE) CSB-YP401935SVC
CSB-EP401935SVC
CSB-BP401935SVC
CSB-MP401935SVC
CSB-EP401935SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L11 (rplK) CSB-YP401936SVC
CSB-EP401936SVC
CSB-BP401936SVC
CSB-MP401936SVC
CSB-EP401936SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP401937SVC
CSB-EP401937SVC
CSB-BP401937SVC
CSB-MP401937SVC
CSB-EP401937SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem I iron-sulfur center (psaC) CSB-YP401938SVB
CSB-EP401938SVB
CSB-BP401938SVB
CSB-MP401938SVB
CSB-EP401938SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ycf48-like protein (SynRCC307_0211) CSB-YP401939SVB
CSB-EP401939SVB
CSB-BP401939SVB
CSB-MP401939SVB
CSB-EP401939SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem I assembly protein ycf3 (ycf3) CSB-YP401940SVB
CSB-EP401940SVB
CSB-BP401940SVB
CSB-MP401940SVB
CSB-EP401940SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S21 (rpsU) CSB-YP401941SVB
CSB-EP401941SVB
CSB-BP401941SVB
CSB-MP401941SVB
CSB-EP401941SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Cell division topological specificity factor (minE) CSB-YP401942SVB
CSB-EP401942SVB
CSB-BP401942SVB
CSB-MP401942SVB
CSB-EP401942SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Cell division protein SepF (sepF) CSB-YP401943SVB
CSB-EP401943SVB
CSB-BP401943SVB
CSB-MP401943SVB
CSB-EP401943SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Elongation factor 4 (lepA), partial CSB-YP401944SVB
CSB-EP401944SVB
CSB-BP401944SVB
CSB-MP401944SVB
CSB-EP401944SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L34 (rpmH) CSB-YP401945SVB
CSB-EP401945SVB
CSB-BP401945SVB
CSB-MP401945SVB
CSB-EP401945SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP401946SVB
CSB-EP401946SVB
CSB-BP401946SVB
CSB-MP401946SVB
CSB-EP401946SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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