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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bovine Glutathione S-transferase P (GSTP1) CSB-YP009989BO
CSB-EP009989BO
CSB-BP009989BO
CSB-MP009989BO
CSB-EP009989BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Receptor-type tyrosine-protein phosphatase mu (Ptprm), partial CSB-YP019058MO
CSB-EP019058MO
CSB-BP019058MO
CSB-MP019058MO
CSB-EP019058MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Cytosol aminopeptidase (LAP3) CSB-YP012745PI
CSB-EP012745PI
CSB-BP012745PI
CSB-MP012745PI
CSB-EP012745PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human 11-beta-hydroxysteroid dehydrogenase 1 (HSD11B1), partial CSB-YP010763HU
CSB-BP010763HU
CSB-MP010763HU
CSB-EP010763HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Gap junction beta-2 protein (GJB2), partial CSB-YP009452HU1
CSB-EP009452HU1
CSB-BP009452HU1
CSB-MP009452HU1
CSB-EP009452HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Hippocalcin-like protein 1 (HPCAL1) CSB-YP010695BO
CSB-EP010695BO
CSB-BP010695BO
CSB-MP010695BO
CSB-EP010695BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat D-beta-hydroxybutyrate dehydrogenase, mitochondrial (Bdh1) CSB-YP002648RA
CSB-EP002648RA
CSB-BP002648RA
CSB-MP002648RA
CSB-EP002648RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Methylated-DNA--protein-cysteine methyltransferase (MGMT) CSB-YP013788BO
CSB-EP013788BO
CSB-BP013788BO
CSB-MP013788BO
CSB-EP013788BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ephrin type-B receptor 2 (EPHB2), partial CSB-YP007730HU
CSB-EP007730HU
CSB-BP007730HU
CSB-MP007730HU
CSB-EP007730HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transketolase (TKT) CSB-YP023580HU
CSB-BP023580HU
CSB-MP023580HU
CSB-EP023580HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Beta-hexosaminidase subunit alpha (Hexa) CSB-YP010315MO
CSB-EP010315MO
CSB-BP010315MO
CSB-MP010315MO
CSB-EP010315MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Serpin H1 (Serpinh1) CSB-YP021087RA
CSB-EP021087RA
CSB-BP021087RA
CSB-MP021087RA
CSB-EP021087RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human RNA-binding motif, single-stranded-interacting protein 1 (RBMS1) CSB-YP019440HU
CSB-EP019440HU
CSB-BP019440HU
CSB-MP019440HU
CSB-EP019440HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse NEDD8 (Nedd8) CSB-YP015684MO
CSB-EP015684MO
CSB-BP015684MO
CSB-MP015684MO
CSB-EP015684MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Elongation factor 1-gamma (EEF1G) CSB-YP007433RB
CSB-EP007433RB
CSB-BP007433RB
CSB-MP007433RB
CSB-EP007433RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Alpha-lactalbumin (Lalba) CSB-YP012724MO
CSB-EP012724MO
CSB-BP012724MO
CSB-MP012724MO
CSB-EP012724MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ornithine aminotransferase, mitochondrial (Oat) CSB-YP016242MO
CSB-EP016242MO
CSB-BP016242MO
CSB-MP016242MO
CSB-EP016242MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Myristoylated alanine-rich C-kinase substrate (MARCKS) CSB-YP013493HU
CSB-EP013493HU
CSB-BP013493HU
CSB-MP013493HU
CSB-EP013493HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse cGMP-gated cation channel alpha-1 (Cnga1), partial CSB-YP005642MO1
CSB-EP005642MO1
CSB-BP005642MO1
CSB-MP005642MO1
CSB-EP005642MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Guanine nucleotide-binding protein subunit alpha-11 (GNA11) CSB-YP009583HU
CSB-EP009583HU
CSB-BP009583HU
CSB-MP009583HU
CSB-EP009583HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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