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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacteroides vulgatus Adenylate kinase (adk) CSB-YP406091BOP
CSB-EP406091BOP
CSB-BP406091BOP
CSB-MP406091BOP
CSB-EP406091BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP406092BOP
CSB-EP406092BOP
CSB-BP406092BOP
CSB-MP406092BOP
CSB-EP406092BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP406093BOP
CSB-EP406093BOP
CSB-BP406093BOP
CSB-MP406093BOP
CSB-EP406093BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus UPF0597 protein BVU_2091 (BVU_2091) CSB-YP406094BOP
CSB-EP406094BOP
CSB-BP406094BOP
CSB-MP406094BOP
CSB-EP406094BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Histidinol-phosphate aminotransferase (hisC) CSB-YP406095BOP
CSB-EP406095BOP
CSB-BP406095BOP
CSB-MP406095BOP
CSB-EP406095BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Dihydroxy-acid dehydratase (ilvD), partial CSB-YP406096BOP
CSB-EP406096BOP
CSB-BP406096BOP
CSB-MP406096BOP
CSB-EP406096BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP406097BOP
CSB-EP406097BOP
CSB-BP406097BOP
CSB-MP406097BOP
CSB-EP406097BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Uronate isomerase (uxaC) CSB-YP406098BOP
CSB-EP406098BOP
CSB-BP406098BOP
CSB-MP406098BOP
CSB-EP406098BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Proline--tRNA ligase (proS) CSB-YP406099BOP
CSB-EP406099BOP
CSB-BP406099BOP
CSB-MP406099BOP
CSB-EP406099BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP406100BOP
CSB-EP406100BOP
CSB-BP406100BOP
CSB-MP406100BOP
CSB-EP406100BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Thiamine-phosphate synthase (thiE) CSB-YP406101BOP
CSB-EP406101BOP
CSB-BP406101BOP
CSB-MP406101BOP
CSB-EP406101BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP406102BOP
CSB-EP406102BOP
CSB-BP406102BOP
CSB-MP406102BOP
CSB-EP406102BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parabacteroides distasonis Arginine repressor (argR) CSB-YP406103PYB
CSB-EP406103PYB
CSB-BP406103PYB
CSB-MP406103PYB
CSB-EP406103PYB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parabacteroides distasonis UPF0059 membrane protein BDI_0173 (BDI_0173), partial CSB-YP406104PYB1
CSB-EP406104PYB1
CSB-BP406104PYB1
CSB-MP406104PYB1
CSB-EP406104PYB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parabacteroides distasonis tRNA dimethylallyltransferase 1 (miaA1) CSB-YP406105PYB
CSB-EP406105PYB
CSB-BP406105PYB
CSB-MP406105PYB
CSB-EP406105PYB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parabacteroides distasonis SsrA-binding protein (smpB) CSB-YP406106PYB
CSB-EP406106PYB
CSB-BP406106PYB
CSB-MP406106PYB
CSB-EP406106PYB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parabacteroides distasonis Tryptophan synthase alpha chain (trpA) CSB-YP406107PYB
CSB-EP406107PYB
CSB-BP406107PYB
CSB-MP406107PYB
CSB-EP406107PYB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parabacteroides distasonis Protein CrcB homolog (crcB), partial CSB-YP406108PYB1
CSB-EP406108PYB1
CSB-BP406108PYB1
CSB-MP406108PYB1
CSB-EP406108PYB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parabacteroides distasonis Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP406109PYB
CSB-EP406109PYB
CSB-BP406109PYB
CSB-MP406109PYB
CSB-EP406109PYB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parabacteroides distasonis Glycosyl hydrolase family 109 protein (BDI_1155) CSB-YP406110PYB
CSB-EP406110PYB
CSB-BP406110PYB
CSB-MP406110PYB
CSB-EP406110PYB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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