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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Clostridium beijerinckii Protein MraZ (mraZ) CSB-YP406171DUC
CSB-EP406171DUC
CSB-BP406171DUC
CSB-MP406171DUC
CSB-EP406171DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii Bifunctional protein FolD (folD) CSB-YP406172DUC
CSB-EP406172DUC
CSB-BP406172DUC
CSB-MP406172DUC
CSB-EP406172DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP406173DUC
CSB-EP406173DUC
CSB-BP406173DUC
CSB-MP406173DUC
CSB-EP406173DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii 33 kDa chaperonin (hslO) CSB-YP406174DUC
CSB-EP406174DUC
CSB-BP406174DUC
CSB-MP406174DUC
CSB-EP406174DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii Biotin synthase (bioB) CSB-YP406175DUC
CSB-EP406175DUC
CSB-BP406175DUC
CSB-MP406175DUC
CSB-EP406175DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii UPF0210 protein Cbei_2352 (Cbei_2352) CSB-YP406176DUC
CSB-EP406176DUC
CSB-BP406176DUC
CSB-MP406176DUC
CSB-EP406176DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii DNA mismatch repair protein MutS (mutS), partial CSB-YP406177DUC
CSB-EP406177DUC
CSB-BP406177DUC
CSB-MP406177DUC
CSB-EP406177DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii UPF0317 protein Cbei_2760 (Cbei_2760) CSB-YP406178DUC
CSB-EP406178DUC
CSB-BP406178DUC
CSB-MP406178DUC
CSB-EP406178DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP406179DUC
CSB-EP406179DUC
CSB-BP406179DUC
CSB-MP406179DUC
CSB-EP406179DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii Flagellar assembly factor FliW (fliW) CSB-YP406180DUC
CSB-EP406180DUC
CSB-BP406180DUC
CSB-MP406180DUC
CSB-EP406180DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP406181DUC
CSB-EP406181DUC
CSB-BP406181DUC
CSB-MP406181DUC
CSB-EP406181DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii Leucine--tRNA ligase (leuS), partial CSB-YP406182DUC
CSB-EP406182DUC
CSB-BP406182DUC
CSB-MP406182DUC
CSB-EP406182DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP406183DUC
CSB-EP406183DUC
CSB-BP406183DUC
CSB-MP406183DUC
CSB-EP406183DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Demansia vestigiata Short neurotoxin SNTX-1 CSB-YP406184DJD
CSB-EP406184DJD
CSB-BP406184DJD
CSB-MP406184DJD
CSB-EP406184DJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Demansia vestigiata Protease inhibitor vestiginin-1 CSB-YP406185DJD
CSB-EP406185DJD
CSB-BP406185DJD
CSB-MP406185DJD
CSB-EP406185DJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Demansia vestigiata Venom nerve growth factor 2 CSB-YP406186DJD
CSB-EP406186DJD
CSB-BP406186DJD
CSB-MP406186DJD
CSB-EP406186DJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Demansia vestigiata Protease inhibitor vestiginin-5 CSB-YP406187DJD
CSB-EP406187DJD
CSB-BP406187DJD
CSB-MP406187DJD
CSB-EP406187DJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buxus microphylla 30S ribosomal protein S2, chloroplastic (rps2) CSB-YP406188BPX
CSB-EP406188BPX
CSB-BP406188BPX
CSB-MP406188BPX
CSB-EP406188BPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buxus microphylla Cytochrome b6-f complex subunit 8 (petN), partial CSB-YP406189BPX
CSB-EP406189BPX
CSB-BP406189BPX
CSB-MP406189BPX
CSB-EP406189BPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buxus microphylla 30S ribosomal protein S14, chloroplastic (rps14) CSB-YP406190BPX
CSB-EP406190BPX
CSB-BP406190BPX
CSB-MP406190BPX
CSB-EP406190BPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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