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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Klebsiella pneumoniae subsp. pneumoniae 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP407612KAX
CSB-EP407612KAX
CSB-BP407612KAX
CSB-MP407612KAX
CSB-EP407612KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Cobyric acid synthase (cobQ), partial CSB-YP407613KAX
CSB-EP407613KAX
CSB-BP407613KAX
CSB-MP407613KAX
CSB-EP407613KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Probable propionate kinase (pduW) CSB-YP407614KAX
CSB-EP407614KAX
CSB-BP407614KAX
CSB-MP407614KAX
CSB-EP407614KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae RNA pyrophosphohydrolase (rppH) CSB-YP407615KAX
CSB-EP407615KAX
CSB-BP407615KAX
CSB-MP407615KAX
CSB-EP407615KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae UPF0267 protein KPN78578_32640 (KPN78578_32640) CSB-YP407616KAX
CSB-EP407616KAX
CSB-BP407616KAX
CSB-MP407616KAX
CSB-EP407616KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae S-adenosylmethionine synthase (metK) CSB-YP407617KAX
CSB-EP407617KAX
CSB-BP407617KAX
CSB-MP407617KAX
CSB-EP407617KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae UPF0235 protein KPN78578_33230 (KPN78578_33230) CSB-YP407618KAX
CSB-EP407618KAX
CSB-BP407618KAX
CSB-MP407618KAX
CSB-EP407618KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae UPF0441 protein KPN78578_33850 (KPN78578_33850) CSB-YP407619KAX
CSB-EP407619KAX
CSB-BP407619KAX
CSB-MP407619KAX
CSB-EP407619KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Urease subunit gamma (ureA) CSB-YP407620KAX
CSB-EP407620KAX
CSB-BP407620KAX
CSB-MP407620KAX
CSB-EP407620KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Urease accessory protein UreF (ureF) CSB-YP407621KAX
CSB-EP407621KAX
CSB-BP407621KAX
CSB-MP407621KAX
CSB-EP407621KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Autoinducer 2 kinase LsrK (lsrK), partial CSB-YP407622KAX
CSB-EP407622KAX
CSB-BP407622KAX
CSB-MP407622KAX
CSB-EP407622KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Penicillin-binding protein activator LpoA (lpoA), partial CSB-YP407623KAX
CSB-EP407623KAX
CSB-BP407623KAX
CSB-MP407623KAX
CSB-EP407623KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 30S ribosomal protein S15 (rpsO) CSB-YP407624KAX
CSB-EP407624KAX
CSB-BP407624KAX
CSB-MP407624KAX
CSB-EP407624KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Sulfoxide reductase heme-binding subunit YedZ (yedZ), partial CSB-YP407625KAX1
CSB-EP407625KAX1
CSB-BP407625KAX1
CSB-MP407625KAX1
CSB-EP407625KAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Ribosomal RNA small subunit methyltransferase B (rsmB) CSB-YP407626KAX
CSB-EP407626KAX
CSB-BP407626KAX
CSB-MP407626KAX
CSB-EP407626KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 50S ribosomal protein L30 (rpmD) CSB-YP407627KAX
CSB-EP407627KAX
CSB-BP407627KAX
CSB-MP407627KAX
CSB-EP407627KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 30S ribosomal protein S7 (rpsG) CSB-YP407628KAX
CSB-EP407628KAX
CSB-BP407628KAX
CSB-MP407628KAX
CSB-EP407628KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 3-dehydroquinate synthase (aroB) CSB-YP407629KAX
CSB-EP407629KAX
CSB-BP407629KAX
CSB-MP407629KAX
CSB-EP407629KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Glycogen synthase (glgA) CSB-YP407630KAX
CSB-EP407630KAX
CSB-BP407630KAX
CSB-MP407630KAX
CSB-EP407630KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD (arnD) CSB-YP407631KAX
CSB-EP407631KAX
CSB-BP407631KAX
CSB-MP407631KAX
CSB-EP407631KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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