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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Sinorhizobium medicae Exodeoxyribonuclease 7 large subunit (xseA), partial CSB-YP409352STV
CSB-EP409352STV
CSB-BP409352STV
CSB-MP409352STV
CSB-EP409352STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae NADH-quinone oxidoreductase subunit D 2 (nuoD2) CSB-YP409353STV
CSB-EP409353STV
CSB-BP409353STV
CSB-MP409353STV
CSB-EP409353STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Probable potassium transport system protein kup 3 (kup3), partial CSB-YP409354STV
CSB-EP409354STV
CSB-BP409354STV
CSB-MP409354STV
CSB-EP409354STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii GMP synthase [glutamine-hydrolyzing] subunit B (guaAB) CSB-YP409355MNS
CSB-EP409355MNS
CSB-BP409355MNS
CSB-MP409355MNS
CSB-EP409355MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Dihydrodipicolinate reductase (dapB) CSB-YP409356MNS
CSB-EP409356MNS
CSB-BP409356MNS
CSB-MP409356MNS
CSB-EP409356MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii UPF0254 protein Mevan_0254 (Mevan_0254) CSB-YP409357MNS
CSB-EP409357MNS
CSB-BP409357MNS
CSB-MP409357MNS
CSB-EP409357MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Anthranilate phosphoribosyltransferase (trpD) CSB-YP409358MNS
CSB-EP409358MNS
CSB-BP409358MNS
CSB-MP409358MNS
CSB-EP409358MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii V-type ATP synthase subunit E (atpE) CSB-YP409359MNS
CSB-EP409359MNS
CSB-BP409359MNS
CSB-MP409359MNS
CSB-EP409359MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii 5'-nucleotidase surE (surE) CSB-YP409360MNS
CSB-EP409360MNS
CSB-BP409360MNS
CSB-MP409360MNS
CSB-EP409360MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii tRNA-splicing endonuclease (endA) CSB-YP409361MNS
CSB-EP409361MNS
CSB-BP409361MNS
CSB-MP409361MNS
CSB-EP409361MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Ribose-5-phosphate isomerase A (rpiA) CSB-YP409362MNS
CSB-EP409362MNS
CSB-BP409362MNS
CSB-MP409362MNS
CSB-EP409362MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Probable 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP409363MNS
CSB-EP409363MNS
CSB-BP409363MNS
CSB-MP409363MNS
CSB-EP409363MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP409364MNS
CSB-EP409364MNS
CSB-BP409364MNS
CSB-MP409364MNS
CSB-EP409364MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii 30S ribosomal protein S11P (rps11p) CSB-YP409365MNS
CSB-EP409365MNS
CSB-BP409365MNS
CSB-MP409365MNS
CSB-EP409365MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii 50S ribosomal protein L22P (rpl22p) CSB-YP409366MNS
CSB-EP409366MNS
CSB-BP409366MNS
CSB-MP409366MNS
CSB-EP409366MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii 50S ribosomal protein L30P (rpl30p) CSB-YP409367MNS
CSB-EP409367MNS
CSB-BP409367MNS
CSB-MP409367MNS
CSB-EP409367MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii 2-phosphoglycerate kinase (pgk2) CSB-YP409368MNS
CSB-EP409368MNS
CSB-BP409368MNS
CSB-MP409368MNS
CSB-EP409368MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii 5-methylthioadenosine/S-adenosylhomocysteine deaminase (mtaD) CSB-YP409369MNS
CSB-EP409369MNS
CSB-BP409369MNS
CSB-MP409369MNS
CSB-EP409369MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Tetrahydromethanopterin S-methyltransferase subunit E (mtrE), partial CSB-YP409370MNS1
CSB-EP409370MNS1
CSB-BP409370MNS1
CSB-MP409370MNS1
CSB-EP409370MNS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Nicotinamide-nucleotide adenylyltransferase (Mevan_0890) CSB-YP409371MNS
CSB-EP409371MNS
CSB-BP409371MNS
CSB-MP409371MNS
CSB-EP409371MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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