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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas aeruginosa Deoxycytidine triphosphate deaminase (dcd) CSB-YP408712PZL
CSB-EP408712PZL
CSB-BP408712PZL
CSB-MP408712PZL
CSB-EP408712PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Probable D-serine dehydratase (dsdA) CSB-YP408713PZL
CSB-EP408713PZL
CSB-BP408713PZL
CSB-MP408713PZL
CSB-EP408713PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Probable molybdopterin-guanine dinucleotide biosynthesis protein A (mobA) CSB-YP408714PZL
CSB-EP408714PZL
CSB-BP408714PZL
CSB-MP408714PZL
CSB-EP408714PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa UPF0434 protein PSPA7_2181 (PSPA7_2181) CSB-YP408715PZL
CSB-EP408715PZL
CSB-BP408715PZL
CSB-MP408715PZL
CSB-EP408715PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Elongation factor P (efp) CSB-YP408716PZL
CSB-EP408716PZL
CSB-BP408716PZL
CSB-MP408716PZL
CSB-EP408716PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Threonine--tRNA ligase (thrS), partial CSB-YP408717PZL
CSB-EP408717PZL
CSB-BP408717PZL
CSB-MP408717PZL
CSB-EP408717PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP408718PZL
CSB-EP408718PZL
CSB-BP408718PZL
CSB-MP408718PZL
CSB-EP408718PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Outer-membrane lipoprotein carrier protein (lolA) CSB-YP408719PZL
CSB-EP408719PZL
CSB-BP408719PZL
CSB-MP408719PZL
CSB-EP408719PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Glycogen synthase (glgA), partial CSB-YP408720PZL
CSB-EP408720PZL
CSB-BP408720PZL
CSB-MP408720PZL
CSB-EP408720PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Serine/threonine transporter sstT (sstT), partial CSB-YP408721PZL
CSB-EP408721PZL
CSB-BP408721PZL
CSB-MP408721PZL
CSB-EP408721PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa NADH-quinone oxidoreductase subunit A 2 (nuoA2), partial CSB-YP408722PZL1
CSB-EP408722PZL1
CSB-BP408722PZL1
CSB-MP408722PZL1
CSB-EP408722PZL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Bifunctional protein FolD (folD) CSB-YP408723PZL
CSB-BP408723PZL
CSB-MP408723PZL
CSB-EP408723PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Chorismate synthase (aroC) CSB-YP408724PZL
CSB-EP408724PZL
CSB-BP408724PZL
CSB-MP408724PZL
CSB-EP408724PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP408725PZL
CSB-EP408725PZL
CSB-BP408725PZL
CSB-MP408725PZL
CSB-EP408725PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa UPF0745 protein PSPA7_4096 (PSPA7_4096) CSB-YP408726PZL
CSB-EP408726PZL
CSB-BP408726PZL
CSB-MP408726PZL
CSB-EP408726PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Flagellar P-ring protein (flgI) CSB-YP408727PZL
CSB-EP408727PZL
CSB-BP408727PZL
CSB-MP408727PZL
CSB-EP408727PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 7-cyano-7-deazaguanine synthase (queC) CSB-YP408728PZL
CSB-EP408728PZL
CSB-BP408728PZL
CSB-MP408728PZL
CSB-EP408728PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa UPF0761 membrane protein PSPA7_4558 (PSPA7_4558), partial CSB-YP408729PZL1
CSB-EP408729PZL1
CSB-BP408729PZL1
CSB-MP408729PZL1
CSB-EP408729PZL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa UPF0176 protein PSPA7_4661 (PSPA7_4661) CSB-YP408730PZL
CSB-EP408730PZL
CSB-BP408730PZL
CSB-MP408730PZL
CSB-EP408730PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa DNA repair protein recO (recO) CSB-YP408731PZL
CSB-EP408731PZL
CSB-BP408731PZL
CSB-MP408731PZL
CSB-EP408731PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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