Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus 30S ribosomal protein S19 (rpsS) CSB-YP408592SUI
CSB-EP408592SUI
CSB-BP408592SUI
CSB-MP408592SUI
CSB-EP408592SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable molybdopterin-guanine dinucleotide biosynthesis protein A (mobA) CSB-YP408593SUI
CSB-EP408593SUI
CSB-BP408593SUI
CSB-MP408593SUI
CSB-EP408593SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Imidazolonepropionase (hutI) CSB-YP408594SUI
CSB-EP408594SUI
CSB-BP408594SUI
CSB-MP408594SUI
CSB-EP408594SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ferredoxin--NADP reductase (SaurJH1_2442) CSB-YP408595SUI
CSB-EP408595SUI
CSB-BP408595SUI
CSB-MP408595SUI
CSB-EP408595SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Biotin synthase (bioB) CSB-YP408596SUI
CSB-EP408596SUI
CSB-BP408596SUI
CSB-MP408596SUI
CSB-EP408596SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP phosphoribosyltransferase (hisG) CSB-YP408597SUI
CSB-EP408597SUI
CSB-BP408597SUI
CSB-MP408597SUI
CSB-EP408597SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Integration host factor subunit beta (ihfB) CSB-YP408598STV
CSB-EP408598STV
CSB-BP408598STV
CSB-MP408598STV
CSB-EP408598STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae tRNA (guanine-N (7)-)-methyltransferase CSB-YP408599STV
CSB-EP408599STV
CSB-BP408599STV
CSB-MP408599STV
CSB-EP408599STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Orotate phosphoribosyltransferase (pyrE) CSB-YP408600STV
CSB-EP408600STV
CSB-BP408600STV
CSB-MP408600STV
CSB-EP408600STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Flagellar hook-basal body complex protein FliE (fliE) CSB-YP408601STV
CSB-EP408601STV
CSB-BP408601STV
CSB-MP408601STV
CSB-EP408601STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Betaine aldehyde dehydrogenase (betB) CSB-YP408602STV
CSB-EP408602STV
CSB-BP408602STV
CSB-MP408602STV
CSB-EP408602STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Quinolinate synthase A (nadA) CSB-YP408603STV
CSB-EP408603STV
CSB-BP408603STV
CSB-MP408603STV
CSB-EP408603STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Transcriptional repressor NrdR (nrdR) CSB-YP408604STV
CSB-EP408604STV
CSB-BP408604STV
CSB-MP408604STV
CSB-EP408604STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Heme A synthase (ctaA), partial CSB-YP408605STV
CSB-EP408605STV
CSB-BP408605STV
CSB-MP408605STV
CSB-EP408605STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP408606STV
CSB-EP408606STV
CSB-BP408606STV
CSB-MP408606STV
CSB-EP408606STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Aspartate carbamoyltransferase (pyrB) CSB-YP408607STV
CSB-EP408607STV
CSB-BP408607STV
CSB-MP408607STV
CSB-EP408607STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae UPF0386 protein Smed_0945 (Smed_0945) CSB-YP408608STV
CSB-EP408608STV
CSB-BP408608STV
CSB-MP408608STV
CSB-EP408608STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae 30S ribosomal protein S3 (rpsC) CSB-YP408609STV
CSB-EP408609STV
CSB-BP408609STV
CSB-MP408609STV
CSB-EP408609STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae 50S ribosomal protein L6 (rplF) CSB-YP408610STV
CSB-EP408610STV
CSB-BP408610STV
CSB-MP408610STV
CSB-EP408610STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Octanoyltransferase (lipB) CSB-YP408611STV
CSB-EP408611STV
CSB-BP408611STV
CSB-MP408611STV
CSB-EP408611STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>