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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Actinobacillus succinogenes 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP410393AUG
CSB-EP410393AUG
CSB-BP410393AUG
CSB-MP410393AUG
CSB-EP410393AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes UPF0283 membrane protein Asuc_0957 (Asuc_0957), partial CSB-YP410394AUG1
CSB-EP410394AUG1
CSB-BP410394AUG1
CSB-MP410394AUG1
CSB-EP410394AUG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP410395AUG
CSB-EP410395AUG
CSB-BP410395AUG
CSB-MP410395AUG
CSB-EP410395AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Outer-membrane lipoprotein carrier protein (lolA) CSB-YP410396AUG
CSB-EP410396AUG
CSB-BP410396AUG
CSB-MP410396AUG
CSB-EP410396AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Ribosome-binding factor A (rbfA) CSB-YP410397AUG
CSB-EP410397AUG
CSB-BP410397AUG
CSB-MP410397AUG
CSB-EP410397AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes UPF0756 membrane protein Asuc_1151 (Asuc_1151), partial CSB-YP410398AUG1
CSB-EP410398AUG1
CSB-BP410398AUG1
CSB-MP410398AUG1
CSB-EP410398AUG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Anthranilate phosphoribosyltransferase (trpD) CSB-YP410399AUG
CSB-EP410399AUG
CSB-BP410399AUG
CSB-MP410399AUG
CSB-EP410399AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes UPF0745 protein Asuc_1390 (Asuc_1390) CSB-YP410400AUG
CSB-EP410400AUG
CSB-BP410400AUG
CSB-MP410400AUG
CSB-EP410400AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Alanine racemase (alr) CSB-YP410401AUG
CSB-EP410401AUG
CSB-BP410401AUG
CSB-MP410401AUG
CSB-EP410401AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP410402AUG
CSB-EP410402AUG
CSB-BP410402AUG
CSB-MP410402AUG
CSB-EP410402AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes GTP cyclohydrolase-2 (ribA) CSB-YP410403AUG
CSB-EP410403AUG
CSB-BP410403AUG
CSB-MP410403AUG
CSB-EP410403AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Protein RnfH (rnfH) CSB-YP410404AUG
CSB-EP410404AUG
CSB-BP410404AUG
CSB-MP410404AUG
CSB-EP410404AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP410405AUG
CSB-EP410405AUG
CSB-BP410405AUG
CSB-MP410405AUG
CSB-EP410405AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP410406AUG
CSB-EP410406AUG
CSB-BP410406AUG
CSB-MP410406AUG
CSB-EP410406AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Uncharacterized protein YjeA (yjeA) CSB-YP410407AUG
CSB-EP410407AUG
CSB-BP410407AUG
CSB-MP410407AUG
CSB-EP410407AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 30S ribosomal protein S15 (rpsO) CSB-YP410408AUG
CSB-EP410408AUG
CSB-BP410408AUG
CSB-MP410408AUG
CSB-EP410408AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP410409AUG
CSB-EP410409AUG
CSB-BP410409AUG
CSB-MP410409AUG
CSB-EP410409AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Probable rRNA maturation factor (Asuc_1945) CSB-YP410410AUG
CSB-EP410410AUG
CSB-BP410410AUG
CSB-MP410410AUG
CSB-EP410410AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes RNA polymerase-associated protein RapA (rapA), partial CSB-YP410411AUG
CSB-EP410411AUG
CSB-BP410411AUG
CSB-MP410411AUG
CSB-EP410411AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 50S ribosomal protein L32 (rpmF) CSB-YP410412AUG
CSB-EP410412AUG
CSB-BP410412AUG
CSB-MP410412AUG
CSB-EP410412AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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