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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Klebsiella pneumoniae subsp. pneumoniae Bifunctional protein FolD (folD) CSB-YP411654KAX
CSB-EP411654KAX
CSB-BP411654KAX
CSB-MP411654KAX
CSB-EP411654KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Lipid A palmitoyltransferase PagP (pagP) CSB-YP411655KAX
CSB-EP411655KAX
CSB-BP411655KAX
CSB-MP411655KAX
CSB-EP411655KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Probable rRNA maturation factor (KPN78578_06730) CSB-YP411656KAX
CSB-EP411656KAX
CSB-BP411656KAX
CSB-MP411656KAX
CSB-EP411656KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Endonuclease 8 (nei) CSB-YP411657KAX
CSB-EP411657KAX
CSB-BP411657KAX
CSB-MP411657KAX
CSB-EP411657KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 8-amino-7-oxononanoate synthase (bioF) CSB-YP411658KAX
CSB-EP411658KAX
CSB-BP411658KAX
CSB-MP411658KAX
CSB-EP411658KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP411659KAX
CSB-EP411659KAX
CSB-BP411659KAX
CSB-MP411659KAX
CSB-EP411659KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Translation initiation factor IF-1 (infA) CSB-YP411660KAX
CSB-EP411660KAX
CSB-BP411660KAX
CSB-MP411660KAX
CSB-EP411660KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP411661KAX
CSB-EP411661KAX
CSB-BP411661KAX
CSB-MP411661KAX
CSB-EP411661KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Cell division inhibitor SulA (sulA) CSB-YP411662KAX
CSB-EP411662KAX
CSB-BP411662KAX
CSB-MP411662KAX
CSB-EP411662KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 50S ribosomal protein L32 (rpmF) CSB-YP411663KAX
CSB-EP411663KAX
CSB-BP411663KAX
CSB-MP411663KAX
CSB-EP411663KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae UPF0259 membrane protein KPN78578_12180 (KPN78578_12180), partial CSB-YP411664KAX1
CSB-EP411664KAX1
CSB-BP411664KAX1
CSB-MP411664KAX1
CSB-EP411664KAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Zinc transport protein ZntB (zntB), partial CSB-YP411665KAX1
CSB-EP411665KAX1
CSB-BP411665KAX1
CSB-MP411665KAX1
CSB-EP411665KAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Spermidine export protein MdtI (mdtI), partial CSB-YP411666KAX1
CSB-EP411666KAX1
CSB-BP411666KAX1
CSB-MP411666KAX1
CSB-EP411666KAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Probable sugar efflux transporter (sotB), partial CSB-YP411667KAX
CSB-EP411667KAX
CSB-BP411667KAX
CSB-MP411667KAX
CSB-EP411667KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 4-hydroxy-2-oxovalerate aldolase (mhpE) CSB-YP411668KAX
CSB-EP411668KAX
CSB-BP411668KAX
CSB-MP411668KAX
CSB-EP411668KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Putative phosphotransferase KPN78578_21280 (KPN78578_21280) CSB-YP411669KAX
CSB-EP411669KAX
CSB-BP411669KAX
CSB-MP411669KAX
CSB-EP411669KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Outer-membrane lipoprotein lolB (lolB) CSB-YP411670KAX
CSB-EP411670KAX
CSB-BP411670KAX
CSB-MP411670KAX
CSB-EP411670KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Endo-type membrane-bound lytic murein transglycosylase A (emtA) CSB-YP411671KAX
CSB-EP411671KAX
CSB-BP411671KAX
CSB-MP411671KAX
CSB-EP411671KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae UPF0260 protein KPN78578_22800 (KPN78578_22800) CSB-YP411672KAX
CSB-EP411672KAX
CSB-BP411672KAX
CSB-MP411672KAX
CSB-EP411672KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae UPF0059 membrane protein KPN78578_23020 (KPN78578_23020), partial CSB-YP411673KAX1
CSB-EP411673KAX1
CSB-BP411673KAX1
CSB-MP411673KAX1
CSB-EP411673KAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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