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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas aeruginosa Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP412833PZL
CSB-EP412833PZL
CSB-BP412833PZL
CSB-MP412833PZL
CSB-EP412833PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP412834PZL
CSB-EP412834PZL
CSB-BP412834PZL
CSB-MP412834PZL
CSB-EP412834PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Protein CyaY (cyaY) CSB-YP412835PZL
CSB-EP412835PZL
CSB-BP412835PZL
CSB-MP412835PZL
CSB-EP412835PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Orotate phosphoribosyltransferase (pyrE) CSB-YP412836PZL
CSB-EP412836PZL
CSB-BP412836PZL
CSB-MP412836PZL
CSB-EP412836PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Probable GTP-binding protein EngB (engB) CSB-YP412837PZL
CSB-EP412837PZL
CSB-BP412837PZL
CSB-MP412837PZL
CSB-EP412837PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Ribonuclease P protein component (rnpA) CSB-YP412838PZL
CSB-EP412838PZL
CSB-BP412838PZL
CSB-MP412838PZL
CSB-EP412838PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ribonuclease Z (rnz) CSB-YP412839MNR
CSB-EP412839MNR
CSB-BP412839MNR
CSB-MP412839MNR
CSB-EP412839MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Translation initiation factor 5A (eIF5A) CSB-YP412840MNR
CSB-EP412840MNR
CSB-BP412840MNR
CSB-MP412840MNR
CSB-EP412840MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Glutamate--tRNA ligase (gltX), partial CSB-YP412841MNR
CSB-EP412841MNR
CSB-BP412841MNR
CSB-MP412841MNR
CSB-EP412841MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis V-type ATP synthase subunit E (atpE) CSB-YP412842MNR
CSB-EP412842MNR
CSB-BP412842MNR
CSB-MP412842MNR
CSB-EP412842MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Bifunctional protein GlmU (MmarC7_0329) CSB-YP412843MNR
CSB-EP412843MNR
CSB-BP412843MNR
CSB-MP412843MNR
CSB-EP412843MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis L-fuculose phosphate aldolase (fucA) CSB-YP412844MNR
CSB-EP412844MNR
CSB-BP412844MNR
CSB-MP412844MNR
CSB-EP412844MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP412845MNR
CSB-EP412845MNR
CSB-BP412845MNR
CSB-MP412845MNR
CSB-EP412845MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis DNA repair and recombination protein radA (radA) CSB-YP412846MNR
CSB-EP412846MNR
CSB-BP412846MNR
CSB-MP412846MNR
CSB-EP412846MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0200 protein MmarC7_0527 (MmarC7_0527) CSB-YP412847MNR
CSB-EP412847MNR
CSB-BP412847MNR
CSB-MP412847MNR
CSB-EP412847MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S12P (rps12p) CSB-YP412848MNR
CSB-EP412848MNR
CSB-BP412848MNR
CSB-MP412848MNR
CSB-EP412848MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0103 protein MmarC7_0632 (MmarC7_0632) CSB-YP412849MNR
CSB-EP412849MNR
CSB-BP412849MNR
CSB-MP412849MNR
CSB-EP412849MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L29P (rpl29p) CSB-YP412850MNR
CSB-EP412850MNR
CSB-BP412850MNR
CSB-MP412850MNR
CSB-EP412850MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L6P (rpl6p) CSB-YP412851MNR
CSB-EP412851MNR
CSB-BP412851MNR
CSB-MP412851MNR
CSB-EP412851MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S5P (rps5p) CSB-YP412852MNR
CSB-EP412852MNR
CSB-BP412852MNR
CSB-MP412852MNR
CSB-EP412852MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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