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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Klebsiella pneumoniae subsp. pneumoniae Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP411674KAX
CSB-EP411674KAX
CSB-BP411674KAX
CSB-MP411674KAX
CSB-EP411674KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae D-cysteine desulfhydrase (dcyD) CSB-YP411675KAX
CSB-EP411675KAX
CSB-BP411675KAX
CSB-MP411675KAX
CSB-EP411675KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Uridine kinase (udk) CSB-YP411676KAX
CSB-EP411676KAX
CSB-BP411676KAX
CSB-MP411676KAX
CSB-EP411676KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Ribonuclease BN (rbn) CSB-YP411677KAX
CSB-EP411677KAX
CSB-BP411677KAX
CSB-MP411677KAX
CSB-EP411677KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP411678KAX1
CSB-EP411678KAX1
CSB-BP411678KAX1
CSB-MP411678KAX1
CSB-EP411678KAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Probable manganese transport protein MntH (mntH), partial CSB-YP411679KAX
CSB-EP411679KAX
CSB-BP411679KAX
CSB-MP411679KAX
CSB-EP411679KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP411680KAX
CSB-EP411680KAX
CSB-BP411680KAX
CSB-MP411680KAX
CSB-EP411680KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Cytoskeleton protein rodZ (rodZ), partial CSB-YP411681KAX
CSB-EP411681KAX
CSB-BP411681KAX
CSB-MP411681KAX
CSB-EP411681KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Elongation factor 4 (lepA), partial CSB-YP411682KAX
CSB-EP411682KAX
CSB-BP411682KAX
CSB-MP411682KAX
CSB-EP411682KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae SsrA-binding protein (smpB) CSB-YP411683KAX
CSB-EP411683KAX
CSB-BP411683KAX
CSB-MP411683KAX
CSB-EP411683KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Alanine--tRNA ligase (alaS), partial CSB-YP411684KAX
CSB-EP411684KAX
CSB-BP411684KAX
CSB-MP411684KAX
CSB-EP411684KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae tRNA pseudouridine synthase D (truD) CSB-YP411685KAX
CSB-EP411685KAX
CSB-BP411685KAX
CSB-MP411685KAX
CSB-EP411685KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae L-fucose isomerase (fucI), partial CSB-YP411686KAX
CSB-EP411686KAX
CSB-BP411686KAX
CSB-MP411686KAX
CSB-EP411686KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Phosphate propanoyltransferase (pduL) CSB-YP411687KAX
CSB-EP411687KAX
CSB-BP411687KAX
CSB-MP411687KAX
CSB-EP411687KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP411688KAX1
CSB-EP411688KAX1
CSB-BP411688KAX1
CSB-MP411688KAX1
CSB-EP411688KAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae UPF0149 protein KPN78578_32810 (KPN78578_32810) CSB-YP411689KAX
CSB-EP411689KAX
CSB-BP411689KAX
CSB-MP411689KAX
CSB-EP411689KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae D-erythrose-4-phosphate dehydrogenase (epd) CSB-YP411690KAX
CSB-EP411690KAX
CSB-BP411690KAX
CSB-MP411690KAX
CSB-EP411690KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae UPF0301 protein KPN78578_33170 (KPN78578_33170) CSB-YP411691KAX
CSB-EP411691KAX
CSB-BP411691KAX
CSB-MP411691KAX
CSB-EP411691KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Probable Fe (2+)-trafficking protein CSB-YP411692KAX
CSB-EP411692KAX
CSB-BP411692KAX
CSB-MP411692KAX
CSB-EP411692KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Bifunctional protein HldE (hldE) CSB-YP411693KAX
CSB-EP411693KAX
CSB-BP411693KAX
CSB-MP411693KAX
CSB-EP411693KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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