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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Sinorhizobium medicae 30S ribosomal protein S17 (rpsQ) CSB-YP413454STV
CSB-EP413454STV
CSB-BP413454STV
CSB-MP413454STV
CSB-EP413454STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae 50S ribosomal protein L15 (rplO) CSB-YP413455STV
CSB-EP413455STV
CSB-BP413455STV
CSB-MP413455STV
CSB-EP413455STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae UPF0229 protein Smed_1028 (Smed_1028) CSB-YP413456STV
CSB-EP413456STV
CSB-BP413456STV
CSB-MP413456STV
CSB-EP413456STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Sec-independent protein translocase protein TatA (tatA), partial CSB-YP413457STV
CSB-EP413457STV
CSB-BP413457STV
CSB-MP413457STV
CSB-EP413457STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae LexA repressor (lexA) CSB-YP413458STV
CSB-EP413458STV
CSB-BP413458STV
CSB-MP413458STV
CSB-EP413458STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP413459STV
CSB-EP413459STV
CSB-BP413459STV
CSB-MP413459STV
CSB-EP413459STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP413460STV
CSB-EP413460STV
CSB-BP413460STV
CSB-MP413460STV
CSB-EP413460STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Urease accessory protein UreG (ureG) CSB-YP413461STV
CSB-EP413461STV
CSB-BP413461STV
CSB-MP413461STV
CSB-EP413461STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae 3-dehydroquinate synthase (aroB) CSB-YP413462STV
CSB-EP413462STV
CSB-BP413462STV
CSB-MP413462STV
CSB-EP413462STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP413463STV
CSB-EP413463STV
CSB-BP413463STV
CSB-MP413463STV
CSB-EP413463STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Dihydroxy-acid dehydratase (ilvD), partial CSB-YP413464STV
CSB-EP413464STV
CSB-BP413464STV
CSB-MP413464STV
CSB-EP413464STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Homogentisate 1,2-dioxygenase (hmgA) CSB-YP413465STV
CSB-EP413465STV
CSB-BP413465STV
CSB-MP413465STV
CSB-EP413465STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae GTPase obg (obg) CSB-YP413466STV
CSB-EP413466STV
CSB-BP413466STV
CSB-MP413466STV
CSB-EP413466STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP413467STV
CSB-EP413467STV
CSB-BP413467STV
CSB-MP413467STV
CSB-EP413467STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Probable intracellular septation protein A (Smed_3090), partial CSB-YP413468STV1
CSB-EP413468STV1
CSB-BP413468STV1
CSB-MP413468STV1
CSB-EP413468STV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae 50S ribosomal protein L32 (rpmF) CSB-YP413469STV
CSB-EP413469STV
CSB-BP413469STV
CSB-MP413469STV
CSB-EP413469STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Protease HtpX homolog (htpX), partial CSB-YP413470STV1
CSB-EP413470STV1
CSB-BP413470STV1
CSB-MP413470STV1
CSB-EP413470STV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Putative phosphotransferase Smed_3209 (Smed_3209) CSB-YP413471STV
CSB-EP413471STV
CSB-BP413471STV
CSB-MP413471STV
CSB-EP413471STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP413472STV
CSB-EP413472STV
CSB-BP413472STV
CSB-MP413472STV
CSB-EP413472STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Chaperone protein DnaK (dnaK), partial CSB-YP413473STV
CSB-EP413473STV
CSB-BP413473STV
CSB-MP413473STV
CSB-EP413473STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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