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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Alkaliphilus metalliredigens Xanthine phosphoribosyltransferase (xpt) CSB-YP413373AUP
CSB-EP413373AUP
CSB-BP413373AUP
CSB-MP413373AUP
CSB-EP413373AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP413374AUP
CSB-EP413374AUP
CSB-BP413374AUP
CSB-MP413374AUP
CSB-EP413374AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Glycine/sarcosine/betaine reductase complex component A (grdA) CSB-YP413375AUP
CSB-EP413375AUP
CSB-BP413375AUP
CSB-MP413375AUP
CSB-EP413375AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Cobalamin synthase (cobS), partial CSB-YP413376AUP1
CSB-EP413376AUP1
CSB-BP413376AUP1
CSB-MP413376AUP1
CSB-EP413376AUP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP413377AUP
CSB-EP413377AUP
CSB-BP413377AUP
CSB-MP413377AUP
CSB-EP413377AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Dihydroorotate dehydrogenase B (NAD (+)), catalytic subunit CSB-YP413378AUP
CSB-EP413378AUP
CSB-BP413378AUP
CSB-MP413378AUP
CSB-EP413378AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens 30S ribosomal protein S13 (rpsM) CSB-YP413379AUP
CSB-EP413379AUP
CSB-BP413379AUP
CSB-MP413379AUP
CSB-EP413379AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens 50S ribosomal protein L23 (rplW) CSB-YP413380AUP
CSB-EP413380AUP
CSB-BP413380AUP
CSB-MP413380AUP
CSB-EP413380AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens 50S ribosomal protein L11 (rplK) CSB-YP413381AUP
CSB-EP413381AUP
CSB-BP413381AUP
CSB-MP413381AUP
CSB-EP413381AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Elongation factor Tu 2 (tuf2) CSB-YP413382AUP
CSB-EP413382AUP
CSB-BP413382AUP
CSB-MP413382AUP
CSB-EP413382AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens UPF0597 protein Amet_4665 (Amet_4665) CSB-YP413383AUP
CSB-EP413383AUP
CSB-BP413383AUP
CSB-MP413383AUP
CSB-EP413383AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Recombination protein RecR (recR) CSB-YP413384AUP
CSB-EP413384AUP
CSB-BP413384AUP
CSB-MP413384AUP
CSB-EP413384AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens tRNA modification GTPase MnmE (mnmE) CSB-YP413385AUP
CSB-EP413385AUP
CSB-BP413385AUP
CSB-MP413385AUP
CSB-EP413385AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Transcriptional regulatory protein walR (walR) CSB-YP413386SUI
CSB-EP413386SUI
CSB-BP413386SUI
CSB-MP413386SUI
CSB-EP413386SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus FMN-dependent NADH-azoreductase (azoR) CSB-YP413387SUI
CSB-EP413387SUI
CSB-BP413387SUI
CSB-MP413387SUI
CSB-EP413387SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) CSB-YP413388SUI
CSB-EP413388SUI
CSB-BP413388SUI
CSB-MP413388SUI
CSB-EP413388SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Xanthine phosphoribosyltransferase (xpt) CSB-YP413389SUI
CSB-EP413389SUI
CSB-BP413389SUI
CSB-MP413389SUI
CSB-EP413389SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Peptidyl-tRNA hydrolase (pth) CSB-YP413390SUI
CSB-EP413390SUI
CSB-BP413390SUI
CSB-MP413390SUI
CSB-EP413390SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative antiporter subunit mnhB2 (mnhB2), partial CSB-YP413391SUI1
CSB-EP413391SUI1
CSB-BP413391SUI1
CSB-MP413391SUI1
CSB-EP413391SUI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 7-cyano-7-deazaguanine synthase (queC) CSB-YP413392SUI
CSB-EP413392SUI
CSB-BP413392SUI
CSB-MP413392SUI
CSB-EP413392SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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