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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methanococcus aeolicus Tryptophan synthase beta chain (trpB) CSB-YP412774MNO
CSB-EP412774MNO
CSB-BP412774MNO
CSB-MP412774MNO
CSB-EP412774MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 3-dehydroquinate synthase (Maeo_1164) CSB-YP412775MNO
CSB-EP412775MNO
CSB-BP412775MNO
CSB-MP412775MNO
CSB-EP412775MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP412776MNO
CSB-EP412776MNO
CSB-BP412776MNO
CSB-MP412776MNO
CSB-EP412776MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Tetrahydromethanopterin S-methyltransferase subunit B (mtrB), partial CSB-YP412777MNO1
CSB-EP412777MNO1
CSB-BP412777MNO1
CSB-MP412777MNO1
CSB-EP412777MNO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP412778MNO
CSB-EP412778MNO
CSB-BP412778MNO
CSB-MP412778MNO
CSB-EP412778MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Cysteine--tRNA ligase (cysS) CSB-YP412779MNO
CSB-EP412779MNO
CSB-BP412779MNO
CSB-MP412779MNO
CSB-EP412779MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 50S ribosomal protein L24P (rpl24p) CSB-YP412780MNO
CSB-EP412780MNO
CSB-BP412780MNO
CSB-MP412780MNO
CSB-EP412780MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 50S ribosomal protein L15P (rpl15p) CSB-YP412781MNO
CSB-EP412781MNO
CSB-BP412781MNO
CSB-MP412781MNO
CSB-EP412781MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP412782MNO
CSB-EP412782MNO
CSB-BP412782MNO
CSB-MP412782MNO
CSB-EP412782MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Protoheme IX farnesyltransferase 1 (cyoE1), partial CSB-YP412783PZL
CSB-EP412783PZL
CSB-BP412783PZL
CSB-MP412783PZL
CSB-EP412783PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Aspartate carbamoyltransferase (pyrB) CSB-YP412784PZL
CSB-EP412784PZL
CSB-BP412784PZL
CSB-MP412784PZL
CSB-EP412784PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 30S ribosomal protein S21 (rpsU) CSB-YP412785PZL
CSB-EP412785PZL
CSB-BP412785PZL
CSB-MP412785PZL
CSB-EP412785PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP412786PZL
CSB-EP412786PZL
CSB-BP412786PZL
CSB-MP412786PZL
CSB-EP412786PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP412787PZL
CSB-EP412787PZL
CSB-BP412787PZL
CSB-MP412787PZL
CSB-EP412787PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP412788PZL
CSB-EP412788PZL
CSB-BP412788PZL
CSB-MP412788PZL
CSB-EP412788PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 50S ribosomal protein L2 (rplB) CSB-YP412789PZL
CSB-EP412789PZL
CSB-BP412789PZL
CSB-MP412789PZL
CSB-EP412789PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 50S ribosomal protein L30 (rpmD) CSB-YP412790PZL
CSB-EP412790PZL
CSB-BP412790PZL
CSB-MP412790PZL
CSB-EP412790PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 50S ribosomal protein L17 (rplQ) CSB-YP412791PZL
CSB-EP412791PZL
CSB-BP412791PZL
CSB-MP412791PZL
CSB-EP412791PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP412792PZL
CSB-EP412792PZL
CSB-BP412792PZL
CSB-MP412792PZL
CSB-EP412792PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Leucine--tRNA ligase (leuS), partial CSB-YP412793PZL
CSB-EP412793PZL
CSB-BP412793PZL
CSB-MP412793PZL
CSB-EP412793PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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