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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cronobacter sakazakii Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP415054CXL
CSB-EP415054CXL
CSB-BP415054CXL
CSB-MP415054CXL
CSB-EP415054CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii DNA protection during starvation protein (dps) CSB-YP415055CXL
CSB-EP415055CXL
CSB-BP415055CXL
CSB-MP415055CXL
CSB-EP415055CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii 50S ribosomal protein L36 2 (rpmJ2) CSB-YP415056CXL
CSB-EP415056CXL
CSB-BP415056CXL
CSB-MP415056CXL
CSB-EP415056CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii FMN reductase (NADH) RutF CSB-YP415057CXL
CSB-EP415057CXL
CSB-BP415057CXL
CSB-MP415057CXL
CSB-EP415057CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP415058CXL
CSB-EP415058CXL
CSB-BP415058CXL
CSB-MP415058CXL
CSB-EP415058CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Pimelyl-[acyl-carrier protein] methyl ester esterase (bioH) CSB-YP415059CXL
CSB-EP415059CXL
CSB-BP415059CXL
CSB-MP415059CXL
CSB-EP415059CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Aspartate 1-decarboxylase (panD) CSB-YP415060CXL
CSB-EP415060CXL
CSB-BP415060CXL
CSB-MP415060CXL
CSB-EP415060CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP415061CXL
CSB-EP415061CXL
CSB-BP415061CXL
CSB-MP415061CXL
CSB-EP415061CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii 30S ribosomal protein S2 (rpsB) CSB-YP415062CXL
CSB-EP415062CXL
CSB-BP415062CXL
CSB-MP415062CXL
CSB-EP415062CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP415063CXL
CSB-EP415063CXL
CSB-BP415063CXL
CSB-MP415063CXL
CSB-EP415063CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Elongation factor 4 (lepA), partial CSB-YP415064CXL
CSB-EP415064CXL
CSB-BP415064CXL
CSB-MP415064CXL
CSB-EP415064CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii UPF0304 protein ESA_00925 (ESA_00925) CSB-YP415065CXL
CSB-EP415065CXL
CSB-BP415065CXL
CSB-MP415065CXL
CSB-EP415065CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Fatty acid oxidation complex subunit alpha (fadJ), partial CSB-YP415066CXL
CSB-EP415066CXL
CSB-BP415066CXL
CSB-MP415066CXL
CSB-EP415066CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Probable lipid kinase YegS-like (ESA_01136) CSB-YP415067CXL
CSB-EP415067CXL
CSB-BP415067CXL
CSB-MP415067CXL
CSB-EP415067CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP415068CXL
CSB-EP415068CXL
CSB-BP415068CXL
CSB-MP415068CXL
CSB-EP415068CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP415069CXL
CSB-EP415069CXL
CSB-BP415069CXL
CSB-MP415069CXL
CSB-EP415069CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Glutamate 5-kinase (proB) CSB-YP415070CXL
CSB-EP415070CXL
CSB-BP415070CXL
CSB-MP415070CXL
CSB-EP415070CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP415071CXL
CSB-EP415071CXL
CSB-BP415071CXL
CSB-MP415071CXL
CSB-EP415071CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Lipoate-protein ligase A (lplA) CSB-YP415072CXL
CSB-EP415072CXL
CSB-BP415072CXL
CSB-MP415072CXL
CSB-EP415072CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Chaperone protein DnaK (dnaK), partial CSB-YP415073CXL
CSB-EP415073CXL
CSB-BP415073CXL
CSB-MP415073CXL
CSB-EP415073CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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