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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Campylobacter curvus Chorismate synthase (aroC) CSB-YP414854CWB
CSB-EP414854CWB
CSB-BP414854CWB
CSB-MP414854CWB
CSB-EP414854CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP414855CWE
CSB-EP414855CWE
CSB-BP414855CWE
CSB-MP414855CWE
CSB-EP414855CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Orotate phosphoribosyltransferase (pyrE) CSB-YP414856CWE
CSB-EP414856CWE
CSB-BP414856CWE
CSB-MP414856CWE
CSB-EP414856CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Polyphosphate kinase (ppk), partial CSB-YP414857CWE
CSB-EP414857CWE
CSB-BP414857CWE
CSB-MP414857CWE
CSB-EP414857CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei tRNA (guanine-N (7)-)-methyltransferase CSB-YP414858CWE
CSB-EP414858CWE
CSB-BP414858CWE
CSB-MP414858CWE
CSB-EP414858CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei UvrABC system protein C (uvrC), partial CSB-YP414859CWE
CSB-EP414859CWE
CSB-BP414859CWE
CSB-MP414859CWE
CSB-EP414859CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei S-ribosylhomocysteine lyase (luxS) CSB-YP414860CWE
CSB-EP414860CWE
CSB-BP414860CWE
CSB-MP414860CWE
CSB-EP414860CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Ribosomal protein L11 methyltransferase (prmA) CSB-YP414861CWE
CSB-EP414861CWE
CSB-BP414861CWE
CSB-MP414861CWE
CSB-EP414861CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Serine/threonine transporter sstT (sstT), partial CSB-YP414862CWE
CSB-EP414862CWE
CSB-BP414862CWE
CSB-MP414862CWE
CSB-EP414862CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Elongation factor 4 (lepA), partial CSB-YP414863CWE
CSB-EP414863CWE
CSB-BP414863CWE
CSB-MP414863CWE
CSB-EP414863CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei UPF0763 protein JJD26997_0796 (JJD26997_0796) CSB-YP414864CWE
CSB-EP414864CWE
CSB-BP414864CWE
CSB-MP414864CWE
CSB-EP414864CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei ATP synthase subunit c (atpE), partial CSB-YP414865CWE1
CSB-EP414865CWE1
CSB-BP414865CWE1
CSB-MP414865CWE1
CSB-EP414865CWE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP414866CWE
CSB-EP414866CWE
CSB-BP414866CWE
CSB-MP414866CWE
CSB-EP414866CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei NH (3)-dependent NAD (+) synthetase CSB-YP414867CWE
CSB-EP414867CWE
CSB-BP414867CWE
CSB-MP414867CWE
CSB-EP414867CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Chaperone protein DnaK (dnaK), partial CSB-YP414868CWE
CSB-EP414868CWE
CSB-BP414868CWE
CSB-MP414868CWE
CSB-EP414868CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP414869CWE
CSB-EP414869CWE
CSB-BP414869CWE
CSB-MP414869CWE
CSB-EP414869CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei 50S ribosomal protein L9 (rplI) CSB-YP414870CWE
CSB-EP414870CWE
CSB-BP414870CWE
CSB-MP414870CWE
CSB-EP414870CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP414871CWE
CSB-EP414871CWE
CSB-BP414871CWE
CSB-MP414871CWE
CSB-EP414871CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP414872CWE
CSB-EP414872CWE
CSB-BP414872CWE
CSB-MP414872CWE
CSB-EP414872CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei 30S ribosomal protein S12 (rpsL) CSB-YP414873CWE
CSB-EP414873CWE
CSB-BP414873CWE
CSB-MP414873CWE
CSB-EP414873CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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