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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Cronobacter sakazakii Fumarate reductase subunit D (frdD), partial CSB-YP415094CXL1
CSB-EP415094CXL1
CSB-BP415094CXL1
CSB-MP415094CXL1
CSB-EP415094CXL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii GTP cyclohydrolase-2 (ribA) CSB-YP415095CXL
CSB-EP415095CXL
CSB-BP415095CXL
CSB-MP415095CXL
CSB-EP415095CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Tyrosine--tRNA ligase (tyrS) CSB-YP415096CXL
CSB-EP415096CXL
CSB-BP415096CXL
CSB-MP415096CXL
CSB-EP415096CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Electron transport complex protein RnfC (rnfC), partial CSB-YP415097CXL
CSB-EP415097CXL
CSB-BP415097CXL
CSB-MP415097CXL
CSB-EP415097CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Protein viaA (viaA) CSB-YP415098CXL
CSB-EP415098CXL
CSB-BP415098CXL
CSB-MP415098CXL
CSB-EP415098CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii D-galactonate dehydratase (dgoD) CSB-YP415099CXL
CSB-EP415099CXL
CSB-BP415099CXL
CSB-MP415099CXL
CSB-EP415099CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP415100CXL
CSB-EP415100CXL
CSB-BP415100CXL
CSB-MP415100CXL
CSB-EP415100CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii UPF0745 protein ESA_01460 (ESA_01460) CSB-YP415101CXL
CSB-EP415101CXL
CSB-BP415101CXL
CSB-MP415101CXL
CSB-EP415101CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Probable Sec-independent protein translocase protein TatE (tatE), partial CSB-YP415102CXL
CSB-EP415102CXL
CSB-BP415102CXL
CSB-MP415102CXL
CSB-EP415102CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii UPF0061 protein ESA_02105 (ESA_02105) CSB-YP415103CXL
CSB-EP415103CXL
CSB-BP415103CXL
CSB-MP415103CXL
CSB-EP415103CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii 50S ribosomal protein L36 1 (rpmJ1) CSB-YP415104CXL
CSB-EP415104CXL
CSB-BP415104CXL
CSB-MP415104CXL
CSB-EP415104CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii 50S ribosomal protein L6 (rplF) CSB-YP415105CXL
CSB-EP415105CXL
CSB-BP415105CXL
CSB-MP415105CXL
CSB-EP415105CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii 50S ribosomal protein L5 (rplE) CSB-YP415106CXL
CSB-EP415106CXL
CSB-BP415106CXL
CSB-MP415106CXL
CSB-EP415106CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (kduI) CSB-YP415107CXL
CSB-EP415107CXL
CSB-BP415107CXL
CSB-MP415107CXL
CSB-EP415107CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP415108CXL
CSB-EP415108CXL
CSB-BP415108CXL
CSB-MP415108CXL
CSB-EP415108CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Cation-efflux pump FieF (fieF), partial CSB-YP415109CXL1
CSB-EP415109CXL1
CSB-BP415109CXL1
CSB-MP415109CXL1
CSB-EP415109CXL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii DNA ligase B (ligB), partial CSB-YP415110CXL
CSB-EP415110CXL
CSB-BP415110CXL
CSB-MP415110CXL
CSB-EP415110CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Ribosome maturation factor RimP (rimP) CSB-YP415111CXL
CSB-EP415111CXL
CSB-BP415111CXL
CSB-MP415111CXL
CSB-EP415111CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Probable UDP-N-acetyl-D-mannosaminuronic acid transferase (wecG) CSB-YP415112CXL
CSB-EP415112CXL
CSB-BP415112CXL
CSB-MP415112CXL
CSB-EP415112CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP415113CXL
CSB-EP415113CXL
CSB-BP415113CXL
CSB-MP415113CXL
CSB-EP415113CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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